摘要
该研究结合了液相-质谱联用技术与转录组分析,系统探讨了刺梨中三萜类化合物的生物合成途径及其调控机制。研究结果表明,在栽培和野生刺梨品种之间,三萜类化合物的含量存在显著差异且栽培品种的三萜类化合物含量比野生品种高出约30 wt.%,且该差异在刺梨的不同生长阶段中均保持稳定。通过高通量转录组测序技术,该研究发现了五个关键基因,它们与三萜类化合物的生物合成密切相关,并与CYP716A1、CPY734A1、CPY72A15、UGT74F2及UGT85A2等基因具有高度同源性,提示它们在刺梨的三萜类化合物合成中可能发挥关键作用。本研究利用代谢组和转录组学的联合分析,不仅明确了刺梨中三萜类化合物的含量差异,还成功锁定了其生物合成的关键基因,为刺梨的品质改良及分子育种提供了新的视角和潜在的分子标记。
In this study,the biosynthesis pathway and regulatory mechanism of triterpenoids in Rosa roxburghii(R.roxburghii)were systematically investigated through combined use of liquid chromatography-mass spectrometry and transcriptome analysis.The results showed that there were significant differences in triterpenoid content between cultivated and wild R.roxburghii varieties,with the cultivated variety exhibiting an approximately 30 wt.%higher triterpenoid content compared with the wild variety.Such a difference remained constant in the different growth stages of R roxburghii.Through high-throughput transcriptome sequencing technology,five key genes associated closely with the biosynthesis of triterpenoid were successfully identified in this research.These genes had high homology to genes such as CYP716A1,CPY734A1,CPY72A15,UGT74F2,and UGT85A2,suggesting their key roles in the synthesis of triterpenoid in R.roxburghii.In this study,the combined metabolomic and transcriptome analysis not only clarified the differences in the triterpenoid content of R.roxburghii and also successfully identified the key genes participating in the biosynthesis of triterpenoids,which offers fresh insights and potential molecular markers for the quality improvement and molecular breeding of R.roxburghii.
作者
何凤金
严艳芳
梁前程
陶仙
李昊东
龙腾相
李良群
邓廷飞
潘雄
陈发菊
杨小生
HE Fengjin;YAN Yanfang;LIANG Qiancheng;TAO Xian;LI Haodong;LONG Tengxiang;LI Liangqun;DENG Tingfei;PAN Xiong;CHEN Faju;YANG Xiaosheng(School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550025,China;Guizhou Natural Products Research Center,State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China)
出处
《现代食品科技》
北大核心
2025年第6期129-136,共8页
Modern Food Science and Technology
基金
国家自然科学基金项目(32160104)
贵州省科技计划项目(黔科合基础ZK[2021]一般512,ZK[2023]一般239
黔科合支撑ZK[2022]一般171,ZK[2022]一般177,[2023]一般472,[2023]一般077)。
关键词
刺梨
三萜类化合物
生物合成
转录组分析
分子育种
Rosa roxburghii
triterpenoids
biosynthesis
transcriptome analysis
molecular breeding
