摘要
每穗粒数是决定水稻产量的重要因素,Gn1a作为主效QTL可通过调控细胞分裂素代谢影响穗粒数。利用CRISPR/Cas9技术在籼稻品种渝金香(YJX WT)Gn1a基因第1外显子上FAD结合结构域编码区进行双靶点敲除,筛选并鉴定获得无外源基因的纯合突变体YJX Gn1a-1。结构域预测表明:与野生型YJX WT相比,YJX Gn1a-1突变体FAD结合结构域缺失26个氨基酸。农艺性状调查显示:突变体植株株高显著增加,一次、二次枝梗数显著增加,每穗粒数显著提升,单株产量显著提高。基因表达分析表明:Gn1a在茎和不同长度幼穗中特异表达,提示其在水稻株高和花序发育中发挥着重要作用。单倍型分析进一步验证了FAD结合结构域的缺失可显著增加水稻株高和穗粒数。
The number of grains per panicle is an important factor determining the yield of rice.Gn1a,as the main effective QTL,can affect the number of grains per panicle by regulating cytokinin metabolism.Double-target knockout was performed in the coding region of the FAD binding domain of exon 1 of the Gn1a gene in indica rice variety Yujinxiang(YJX WT)using CRISPR/Cas9 technology.The homozygous editing mutant YJX Gn1a-1 without exogenous genes was screened and identified.Domain prediction indicates that compared with the wild-type YJX WT,the FAD binding domain of the YJX Gn1a-1 mutant is missing 26 amino acids.The investigation of agronomic traits showed that the height of the mutant plants and the yield per plant were increased significantly,and the number of primary and secondary branches,the number of grains per panicle were increased.Gene expression analysis indicated that Gn1a was specifically expressed in culm and young panicles of different lengths,suggesting that it plays an important role in the plant height and inflorescence development of rice.Haplotype analysis further verified that the absence of the FAD binding domain could significantly increase the plant height and the number of grains per panicle of rice.
作者
赵晓玉
金笛
蒋雨桐
杨朝越
彭柏然
刘晓
王柳静
李云峰
庄慧
ZHAO Xiaoyu;JIN Di;JIANG Yutong;YANG Chaoyue;PENG Boran;LIU Xiao;WANG Liujing;LI Yunfeng;ZHUANG Hui(Rice Research Institute,Southwest University/Academy of Agricultural Sciences/Key Laboratory of Application and Safety Control of Genetically Modified Crops,Chongqing 400715,China)
出处
《西南大学学报(自然科学版)》
北大核心
2025年第7期70-81,共12页
Journal of Southwest University(Natural Science Edition)
基金
重庆市现代农业产业技术体系项目(CQMAITS202301)
国家自然科学基金青年科学基金项目(32100287)
国家自然科学基金项目(31971919,32172044)
重庆市自然科学基金项目(cstc2020jcyj-jqX0020,cstc2021ycjh-bgzxm0066)。
