摘要
探究甜叶菊根多糖(Stevia rebaudiana root polysaccharide, SRRP)对肥胖小鼠炎症反应的影响及其可能机制。采用高脂饮食(high-fat diet, HFD)构建肥胖模型。将肥胖小鼠分为模型组、SRRP高、中、低剂量给药组和阳性药组,每组给予相同体积的生理盐水、SRRP或阿托伐他汀钙(atorvastatin calcium, ATV),连续8周。生化法检测血清中脂质水平和氧化应激水平。ELISA法检测肝脏中炎症因子水平。HE染色法对肝脏和附睾脂肪进行组织学分析。Western blot检测磷酸肌醇3-激酶(phosphoinositide 3-kinase, PI3K)、磷酸化磷酸肌醇3-激酶(p-PI3K)、蛋白激酶B(protein kinase B,AKT)、磷酸化蛋白激酶B(p-AKT)和核因子κB(nuclear factor kappa B,NF-κB)蛋白表达。结果表明,与正常组相比,HFD组小鼠体重明显增加(P<0.01)。HE染色结果提示肝脏内有大量脂肪空泡产生,附睾白色脂肪组织细胞体积变大。血脂水平、氧化应激程度和炎症因子水平显著提高(P<0.01)。与HFD组相比,SRRP和ATV可以降低肥胖小鼠血脂水平、氧化应激程度和炎症因子水平(P<0.01),下调p-PI3K、p-AKT和NF-κB蛋白表达的水平(P<0.01),改善肝脏脂质堆积,减小肥胖小鼠附睾白色脂肪组织细胞体积。由此可见,SRRP可以改善肥胖小鼠血脂水平、肝脏脂质堆积和炎症反应,可能与抑制PI3K/AKT/NF-κB信号通路有关。
This study aimed to investigate the effects of Stevia rebaudiana root polysaccharide(SRRP)on inflammatory responses in obese mice and explore its potential mechanisms.An obesity model was established using a high-fat diet(HFD).Obese mice were divided into a model group,SRRP high-dose group,SRRP medium-dose group,SRRP low-dose group,and a positive control group(atorvastatin calcium,ATV).Each group was administered an equal volume of normal saline,SRRP or ATV for eight consecutive weeks.Serum lipid levels and oxidative stress were measured using biochemical assays.Liver inflammatory factor levels were detected by ELISA.Histological analysis of liver and epididymal adipose tissue was performed using HE staining.Western blot was used to detect the expression levels of phosphoinositide 3-kinase(PI3K),phosphorylated phosphoinositide 3-kinase(p-PI3K),protein kinase B(AKT),phosphorylated protein kinase B(p-AKT),and nuclear factor kappa B(NF-κB).The results showed that compared to the normal group,the body weight of the HFD group significantly increased(P<0.01).HE staining revealed the presence of numerous lipid vacuoles in the liver and enlarged epididymal white adipose tissue cells in the HFD group.Serum lipid levels,oxidative stress,and inflammatory factor levels were significantly elevated in the HFD group(P<0.01).Compared to the HFD group,SRRP and ATV significantly reduced serum lipid levels,oxidative stress,and inflammatory factor levels in obese mice(P<0.01),downregulated the expression levels of p-PI3K,p-AKT,and NF-κB proteins(P<0.01),alleviated hepatic lipid accumulation,and reduced the size of epididymal white adipose tissue cells.These findings suggest that SRRP can improve lipid levels,hepatic lipid accumulation,and inflammatory responses in obese mice,and its mechanism may be related to the inhibition of the PI3K/AKT/NF-κB signaling pathway.
作者
鲍玉龙
柳春燕
车慧
吕秋月
周心月
邵太丽
王国栋
BAO Yu-long;LIU Chun-yan;CHE Hui;LYU Qiu-yue;ZHOU Xin-yue;SHAO Tai-li;WANG Guo-dong(School of Pharmacy,Wannan Medical College;Anhui Provincial Engineering Research Center for Polysaccharide Drugs;Anhui Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicine in Southern;Anhui Innovative Center for Drug Basic Research of Metabolic Diseases,Wuhu 241002,China)
出处
《天然产物研究与开发》
北大核心
2025年第6期1004-1011,共8页
Natural Product Research and Development
基金
安徽省高校自然科学研究重大项目(KJ2021ZD0101)
安徽省高等学校自然科学基金(2024AH051923)
安徽省高校优秀青年科研项目(2022AH030124)
安徽省大学生创新创业训练计划(S202410368083)。
