摘要
目的对子痫前期(PE)相关的miR-943靶基因进行生物信息学分析,为miR-943在子痫前期发生机制中的研究提供新方向。方法从基因表达数据库(GEO)中数据集GSE206988中筛选出在子痫前期患者的胎盘组织中表达显著下调的miR-943,利用miRPathDB、TargetScan数据库进行靶基因预测,并通过韦恩图取其交集数据,利用clusterProfiler R包行靶基因的基因本体(GO)功能注释和京都基因与基因组百科全书(KEGG)通路富集分析,应用数据库STRING构建靶基因的蛋白质相互作用网络(PPI)。结果TargetScan和miRPathDB同时预测获得的靶基因共36个。GO功能注释共富集到15项,细胞组分(CC)主要富集于信号识别颗粒和内质网,分子功能(MF)主要富集于胎盘生长因子激活受体活性、蛋白磷酸酶调节活性等。KEGG通路富集分析显示,miR-943靶基因主要与PI3K-Akt、cAMP、MAPK等信号通路有关。结论miR-943的靶基因功能与胎盘生长因子激活受体活性、蛋白磷酸酶调节活性等有关,参与的信号通路与PE关系密切,miR-943可能通过对靶基因的调控参与到PE的发生发展。
Objective:To conduct bioinformatics analysis of miR-943 target genes related to preeclampsia,so as to provide academic direction for the study of miR-943 in the pathogenesis of preeclampsia.Methods:Based on the dataset GSE206988 in the public gene chip database GEO,which shows downregulation of miR-943 expression in placental tissue of preeclampsia patients.The corresponding analysis websites miRPathDB and TargetScan were used for target gene prediction.The Venn diagram helped derive intersecting data.Gene ontology(GO)functional annotation analysis was conducted to predict possible target genes with clusterProfiler R package,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis was carried out to analyze the concentrate signaling pathway,and target gene protein interaction network(PPI)was constructed with the database STRING.Results:TargetScan and miRPathDB predicted 36 crossover target genes.GO functional annotations were enriched to 15 items,with cellular components(CC)mainly enriched in signal recognition granules and endoplasmic reticulum,and molecular functions(MF)mainly enriched in placental growth factor activated receptor activity,protein phosphatase regulatory activity and so on.KEGG pathway analysis showed that the miR-943 target gene was mainly related to signaling pathways such as PI3K Akt,cAMP,MAPK and so on.Conclusions:The target gene function of miR-943 was related to the activation of placental growth factor receptor activity,protein phosphatase regulation activity and so on.The signaling pathways involved were closely related to PE,and miR-943 may be involved in the occurrence and development of PE through the regulation of target genes.
作者
沈婕
沈树娜
林元
邓森灵
郑华玲
SHEN Jie;SHEN Shu-na;LIN Yuan;DENG Sen-ling;ZHENG Hua-ling(Obstetrics Department,Hainan West Central Hospital,Danzhou 571700)
出处
《生殖医学杂志》
2025年第6期826-832,共7页
Journal of Reproductive Medicine
基金
海南省自然科学基金(822RC853)。
