摘要
目的 探索老年骨质疏松性脊椎压缩性骨折(osteoporotic vertebral compression fracture, OVCF)患者血清外泌体(Exo)-miRNAs表达特征,构建血清Exo-miRNAs标志物用于评估OVCF风险。方法 选择新余市中医院2021年2月至2024年2月收治的211例OVCF患者和232例骨质疏松症(osteoporosis, OP)患者作为研究对象,并选择医院正常体检的74名健康者作为对照组。对OP、OVCF患者及对照组血清Exo-miRNAs通量测序分析寻找差异表达Exo-miRNAs。利用定量实时聚合酶链反应(RT-qPCR)检测血清Exo-miRNAs表达水平。使用R包multiMiR和Venn图分析miRNAs靶基因并进行基因本体论(GO)、京都基因和基因组(KEGG)及WikiPathway信号通路分析。运用受试者工作特征(ROC)曲线评估Exo-miRNAs标志物预测OVCF风险的临床价值。结果 Exo-miR-4522、miR-1469和miR-4739在对照组、OP和OVCF患者中呈现阶梯式递增(P<0.05)。ROC曲线分析结果显示,血清Exo-miR-4522、miR-1469和miR-4739能有效预测OVCF风险,其曲线下面积(AUC)值均>0.7。3个miRNAs靶基因功能主要富集在库欣综合征、胰岛素抵抗、PI3K-Akt和甲状腺素产生与外周下游信号传导效应信号通路中。联合ROC曲线构建血清Exo-miR-4522、miR-1469和miR-4739标志物,相较于对照组和OP患者,该标志物预测OVCF风险的AUC分别为0.997(0.968~1.000)和0.944(0.890~0.976),可显著提高OVCF诊断性能。结论 血清Exo-miR-4522、miR-1469和miR-4739可能参与OVCF发病机制。血清Exo-miRNAs标志物是预测OVCF风险的新型生物标志物。
Objective To explore the expression characteristics of serum exosomes(Exo)-miRNAs in elderly osteoporotic vertebral compression fracture(OVCF)patients and to construct serum Exo-miRNAs maker for assessing OVCF risk.Methods 211 patients with OVCF and 232 patients with osteoporosis(OP)admitted to our hospital in the year February 2023-February 2024 were selected as study subjects,and 74 healthy individuals with normal physical examination in our hospital were selected as the control group.Serum Exo-miRNAs flux sequencing analysis of OP and OVCF patients and controls was performed to look for differentially expressed Exo-miRNAs.The expression levels of serum Exo-miRNAs were detected using quantitative real-time polymerase chain reaction(RT-qPCR).miRNAs target genes were analyzed and subjected to gene ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and WikiPathway signaling pathway analysis using the R package multiMiR and Venn diagrams.The clinical value of Exo-miRNAs maker for predicting OVCF risk was assessed using receiver operating characteristic(ROC)curves.Results Exo-miR-4522,miR-1469 and miR-4739 showed a stepwise increase in control,OP and OVCF patients(P<0.05).ROC curve analysis showed that serum Exo-miR-4522,miR-1469 and miR-4739 were effective in predicting the risk of OVCF,and all of them had area under the curve(AUC)values>0.7.The target gene functions of the three miRNAs were mainly enriched in Cushing's syndrome,insulin resistance,PI3K-Akt and thyroid hormone production and peripheral downstream signaling effects signaling pathways.The combined ROC curves constructed serum Exo-miR-4522,miR-1469 and miR-4739 maker,which predicted OVCF risk with AUC of 0.997(0.968-1.000)and 0.944(0.890-0.976),respectively,in comparison with control and OP patients,and could significantly improve the diagnostic performance of OVCF.Conclusion Serum Exo-miR-4522,miR-1469 and miR-4739 may be involved in OVCF pathogenesis.maker of serum Exo-miRNAs as novel biomarkers for predicting OVCF risk.
作者
黎细平
廖勇
刘翔
LI Xiping;LIAO Yong;LIU Xiang(Xinyu Hospital of Traditional Chinese Medicine,Xinyu 338000,China)
出处
《中国骨质疏松杂志》
北大核心
2025年第5期676-682,共7页
Chinese Journal of Osteoporosis
基金
江西省卫生健康委科技计划项目(202140640)。
