摘要
目的:利用网络药理学方法探讨玉屏风散治疗口腔扁平苔藓(oral lichen planus,OLP)的主要活性成分及作用机制。方法:利用中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)和人类基因数据库(the human gene database,GeneCards)获取玉屏风散的活性成分、作用靶点以及OLP疾病靶点;利用韦恩图分析获得药物与疾病共同靶点;利用STRING软件构建药物与疾病共同靶点蛋白-蛋白互作(protein-protein interactions,PPI)网络,并筛选关键靶点。通过基因功能注释数据库(the database for annotation visualization and integrated discovery,DAVID)对核心靶基因关键靶点进行基因本体论(gene ontology,GO)功能、京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。构建“药物-成分-靶点-通路”网络,获得主要活性成分,并将主要活性成分与关键靶点进行分子对接。结果:共筛选得到关键靶点11个,主要活性成分为槲皮素、汉黄芩素、山柰酚、芒柄花黄素、β-谷甾醇。富集分析发现正向调控RNA聚合酶Ⅱ启动子转录、促进一氧化氮生物合成、Toll样受体、MAPK及T细胞受体介导的信号通路等与OLP相关。分子对接结果表明主要活性成分与关键靶点有一定作用力。结论:玉屏风散治疗OLP具有多靶点、多通路等特点,其可能通过抑制机体炎性反应,改善免疫功能,抑制OLP癌变,发挥治疗OLP的作用。
Objective:To discuss the main active ingredients and mechanism of Yupingfeng powder in the treatment of oral lichen planus(OLP)using network pharmacology.Methods:TCMSP and GeneCards were applied to obtain the active ingredients,targets of action of Yupingfeng powder and OLP-related targets,Venn diagram was utilized to analyze and gain shared targets between medicine and disease.STRING software was used to construct PPI of the shared targets between medicine and disease for the screening of key targets.Go and KEGG of the key targets and core target genes were conducted using DAVID.The network of"medicine-ingredient-target-pathway"was built to obtain the main active ingredients,and to perform molecular docking of the main active ingredients and key targets.Results:All 11 key targets were screened and obtained in the study,the main active ingredients were quercetin,baicalin,kaempferol,flavanthone andβ-glucosinolide.The results of enrichment analysis showed that these factors including the transcription of positive regulation of RNA polymerase II promoter,promoting nitric oxide biosynthesis,toll-like receptor,MAPK and T cell receptor mediated signaling pathways,were closely related to OLP.The results of molecular docking demonstrated that there was a certain interaction between the main active ingredients and key targets.Conclusion:Yupingfeng powder in treating OLP,possessing the characteristics of multiple targets and multiple pathways,could exert therapeutic effects of OLP possibly through inhibiting inflammatory reaction,improving immunologic function and restraining OLP carcinogenesis.
作者
唐伟丽
鲁毅
韩欣妍
李崇
赵芳
郭鹏琦
TANG Weili;LU Yi;HAN Xinyan;LI Chong;ZHAO Fang;GUO Pengqi(Key laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,Hospital of Stomatology,Xi´an Jiaotong University,Xi´an 710004,China;Department of Pharmacy,Northwest Women´s and Children´s Hospital,Xi´an 710061,China;School of Chemical Engineering,Northwest University,Xi´an 710069,China)
出处
《西部中医药》
2025年第5期88-93,共6页
Western Journal of Traditional Chinese Medicine
基金
国家自然科学基金青年科学基金(82000284)
陕西省自然科学基金(2020JQ-609)
中国博士后科学基金(2020M683701XB)
西安交通大学口腔医院青年科研基金项目(201704)。
关键词
口腔扁平苔藓
网络药理学
机制
玉屏风散
成分
oral lichen planus
network pharmacology
mechanism
Yupingfeng powder
ingredients
