摘要
目的筛选口腔扁平苔藓组织中的差异表达基因,并对其进行功能分类。方法用4000种人类基因多聚酶链反应产物制成BiostarH-40s型表达谱芯片,分离纯化正常口腔黏膜组织和口腔扁平苔藓病变组织mRNA,制备表达谱探针,用ScanArray4000荧光扫描仪扫描芯片荧光信号图像,分析正常口腔黏膜组织和口腔扁平苔藓组织之间差异表达的基因。结果1)在4000条基因中,有213条基因表达差异,其中122条基因表达上调,91条基因表达下调。2)在表达上调基因中,功能分类主要包括免疫相关基因、代谢相关基因、癌基因、细胞因子、细胞信号和传递蛋白。3)在表达下调基因中,功能分类主要包括DNA结合、转录和转录因子、细胞信号和传递蛋白、免疫相关基因、细胞因子、代谢相关基因。结论口腔扁平苔藓的发生、发展过程中存在着多条不同功能基因表达调控的改变。
Objective To detect the differentially expressed genes in oral lichen planus（OLP） by eDNA microarray. Methods The PCR products of 4 000 genes were spotted on chemical material coated glass plates in array. The total RNAs were isolated from OLP and normal oral mucosa tissue and were purified to mRNA. Both the mRNA were reversely transcribed to cDNA with the incorporations of fluorescent dUTP, for preparing the hybridization probes. The mixed probes were then hybridized to the cDNA microarray. Microarray was scanned for the fluorescent signals and showed the differences between the two samples. Results The expressions of 122 genes were up regulated while the expressions of 91 genes were down regulated in OLP among the 4 000 target genes. The upregnlated genes were mainly the ones of immunity related genes, metabolize related genes, oncogene, cytokine and cell signal transduction protein. The down-regnlated genes were mainly the ones of DNA binding and transcription factors, cell signal transduction protein, immunity related genes, metabolize related genes, and cytokine. Condusion Two hundred and thirteen differentially expressed genes with different functions were revealed in OLP, which may play some roles in the progression of OLP.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2007年第4期378-382,共5页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(30472123)
江苏省教育厅基金资助项目(04KJD320085)
关键词
口腔扁平苔藓
基因表达谱
基因芯片
oral lichen planus
gene expression profile： gene array
