摘要
以红景天为原料,采用酶解-超声复合法提取红景天多糖。以多糖得率为指标,在单因素试验的基础上通过响应面法优化提取工艺,并对红景天多糖的抗氧化活性进行分析。结果表明:提取红景天多糖的最佳工艺条件为液料比23∶1(mL/g)、酶解时间79 min、酶解温度52℃、pH 4.2、超声时间45 min,在此条件下红景天多糖的得率为9.12%。抗氧化试验结果显示,红景天多糖对DPPH、ABTS自由基均具有较好的清除能力,表明其具有较强的抗氧化能力。
Using Rhodiola crenulata as raw material,Rhodiola crenulata polysaccharides was extracted by enzymatic hydrolysis-ultrasound composite method.Taking polysaccharide yield as the index,the extraction process was optimized through response surface methodology based on single factor experiments,and the antioxidant activity of Rhodiola crenulata polysaccharides was analyzed.The results showed that the optimal conditions were liquid-to-solid ratio 23:1(mL/g),enzymatic hydrolysis time 79 min,enzymatic hydrolysis temperature 52℃,pH 4.2,and ultrasound time 45 min.Under these conditions,the yield of Rhodiola crenulata polysaccharide was 9.12%.The antioxidant tests showed that Rhodiola crenulata polysaccharides had good scavenging ability for DPPH and ABTS free radicals,indicating that it had strong antioxidant capacity.
作者
董佳璇
王莹
李逸群
伊薇晓
吴宗耀
杨然
DONG Jiaxuan;WANG Ying;LI Yiqun;YI Weixiao;WU Zongyao;YANG Ran(School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;Tibetan Medicine Research Institute,Xizang University of Tibetan Medicine,Lhasa 850000,Xizang,China)
出处
《粮食与油脂》
北大核心
2025年第6期110-115,130,共7页
Cereals & Oils
基金
中央引导地方科技发展资金项目(XZ202301YD0023C)
国家现代农业产业技术体系项目(CARS-21)
山东省重点研发计划(2021CXGC010510)
泰山学者工程专项经费资助项目(tsqn202306187)。
关键词
红景天多糖
酶解-超声
工艺优化
响应面法
抗氧化活性
Rhodiola crenulata polysaccharide
enzymatic hydrolysis-ultrasound
process optimization
response surface methodology
antioxidant activity
