摘要
构建负载铁源的水凝胶递送载体是解决肽铁螯合物胃消化稳定性的重要策略。以海藻酸钠和马铃薯淀粉为原料,以氯化钙为交联剂,采用外凝胶法制备了3种负载不同铁源[蛋黄肽铁螯合物(EYP-Fe)、右旋糖酐铁和FeSO_(4)]的水凝胶珠。结果表明:使用质量浓度为1.5 g/100 mL的海藻酸钠和1.0 g/100 mL的马铃薯淀粉混合溶液制备的水凝胶珠,具有较好的成型效果和稳定性,并且对不同铁源的包封率可达84%以上。水凝胶珠在酸性环境中表现出较高的稳定性,溶胀率低于10%;而在中性至弱碱性条件下,水凝胶珠展示出显著的溶胀性能。说明铁元素可在胃部避免沉淀,在肠道中有效释放并被吸收。红外光谱和X射线衍射谱结果显示,亲水性相互作用是水凝胶珠形成的主要驱动力。模拟体外消化过程发现水凝胶珠在胃中发生收缩(29%~31%),铁释放率维持在11.0%~19.0%;其中,负载EYP-Fe的水凝胶珠的铁释放率最低,为11.2%±2.9%。在肠道消化中,水凝胶珠发生溶胀,并缓慢释放其包封的铁源,铁释放率可达79%以上。因此,海藻酸钠/马铃薯淀粉复合水凝胶珠可改善EYP-Fe在胃中的稳定性,同时具备向肠道环境输送铁的能力。研究旨在为新型口服补铁剂的开发提供理论和技术参考。
The construction of iron loaded hydrogel delivery carrier is an important strategy to solve the gastric digestion stability of peptide-iron chelate.Sodium alginate and potato starch were used as raw materials,and calcium chloride was used as cross-linking agent to prepare hydrogel beads loaded with different iron sources[yolk peptide-iron chelate(EYP-Fe),iron dextran and FeSO_(4)]by external gel method.The results showed that the hydrogel beads prepared with 1.5 g/100 mL sodium alginate and 1.0 g/100 mL potato starch mixed solution had good molding effect and stability,and the encapsulation efficiency for different iron sources could reach more than 84%.The hydrogel beads showed high stability in acid environment,with swelling rate less than 10%.Under neutral to weak alkaline conditions,hydrogel beads showed significant swelling properties.This indicated that iron could prevent precipitation in the stomach and be effectively released and absorbed in the intestine.Infrared spectrum and X-ray diffraction results showed that hydrophilic interaction was the main driving force for the formation of hydrogel beads.The simulated in vitro digestion process indicated that the hydrogel beads contracted in the stomach(29%-31%),and the iron release rate was maintained at 11.0%-19.0%.Among them,the EYP-Fe loaded hydrogel beads exhibited the lowest iron release rate(11.2%±2.9%).During intestinal digestion,hydrogel beads swelled and slowly released encapsulated iron sources,with an iron release rate of more than 79%.Therefore,sodium alginate/potato starch composite hydrogel beads could improve the stability of EYP-Fe in the stomach and had the ability to deliver iron to the intestinal environment.The research aimed to provide theoretical and technical references for the development of new oral iron supplements.
作者
向聪
韦帅帅
马璐璐
李述刚
霍嘉颖
XIANG Cong;WEI Shuaishuai;MA Lulu;LI Shugang;HUO Jiaying(Guangzhou Technician College,Guangzhou 510410,China;School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China)
出处
《食品科学技术学报》
北大核心
2025年第3期46-56,66,共12页
Journal of Food Science and Technology
基金
安徽省自然科学基金青年项目(2308085QC111)
“十四五”国家重点研发项目(2022YFD2101001)
“十四五”国家蛋鸡体系项目(JZ2024GQBK0161)
泓星优鹑食品海安有限公司合作项目(W2022JSKF0564)
动物源食品绿色制造与资源挖掘安徽省重点实验室重点项目(PA2023GDSK0125)
安徽荣达食品有限公司合作项目(W2020JSKF0489)。
关键词
肽铁螯合物
水凝胶珠
耐酸性
递送载体
结构表征
模拟消化
peptide-iron chelate
hydrogel beads
acid resistance
delivery carrier
structural characterization
simulated digestion
