摘要
为提高紫苏地方种质资源的利用效率,对来自甘肃的5个栽培区的127份紫苏地方种质资源,利用SSR分子标记技术进行遗传多样性分析。根据127份资源的UPGMA聚类结果,应用位点优先取样策略,并结合采集地信息开展核心种质构建。结果表明,甘肃地方紫苏种质资源具有较低水平的遗传多样性,Nei’s基因多样性指数(H)为0.2366,Shannon多样性指数(I)为0.3724,陇南和天水、平凉和庆阳之间分别具有更高的亲缘关系。最终获得由38份紫苏地方材料组成的核心种质。利用均值、方差、极差、变异系数等指标对紫苏核心种质的7个表型性状、5个油用品质性状检验,表明所构建的核心种质具有较好的代表性和遗传多样性。
To better utilize perilla collection resources,genetic diversities of 127 accessions of perilla(Perilla frutescens(L.)Britt.)from 5 cultivated regions of Gansu were analyzed with SSR markers.Based on result of UPGMA,the accessions were characterized using an allele preferred sampling strategy and geographical information to establish a core collection.Results showed that Nei's gene diversity indexes was 0.2366,Shannon’s information index was 0.3724.In addition,UPGMA cluster analysis also revealed closer genetic relationships between Longnan and Tianshui population,as well as Pingliang and Qingyang population.Genetic distances between groups were associated with their geographical origin.Totally 38 accessions were constituted a core collection.7 phenotypic traits and 5 oil quality traits of the core collection were tested by mean value,variance,range,and coefficient variation,which showed that the constructed core collection had well representativeness and genetic diversity.
作者
崔文娟
罗俊杰
陈军
欧巧明
CUI Wen-juan;LUO Jun-jie;CHEN Jun;OU Qiao-ming(Bio-technology Institute of Gansu Academy of Agricultural Sciences,Lanzhou 730070,China)
出处
《中国油料作物学报》
北大核心
2025年第2期380-392,共13页
Chinese Journal of Oil Crop Sciences
基金
甘肃省农业科学院重点研发项目(2019GAAS15)
甘肃省科技厅支撑计划项目(21CX6NA125)。
关键词
紫苏
种质资源
SSR
遗传多样性
核心种质
Perilla frutescens
germplasms
SSR
genetic diversity
core collection
