摘要
为筛选优异紫苏种质资源及优良育种亲本,提高紫苏育种效率,本研究以159份油用紫苏种质资源为试验材料,对不同紫苏种质11个主要农艺性状及5个关键品质特性进行了鉴定、变异分析、主成分及系统聚类分析等多元统计分析与评价。结果表明,供试紫苏种质主要农艺性状及品质特征的变异系数介于2.60%~35.42%之间,各种质间主要农艺性状及品质特性遗传变异丰富,类型广泛,选择利用的前景较好;主成分分析将主要农艺性状及品质特征聚为6个主成分,即分枝数因子、α-亚麻酸因子、油酸因子、生育期因子、千粒重因子和产量因子,总变异贡献率分别为40.943%、21.549%、12.332%、5.810%、4.486%和4.243%,累计贡献率为89.36%,可代表所考察性状的大部分信息;系统聚类将供试材料聚为五大类群,表明不同类群材料间各农艺性状及品质特征存在较明显的特异性;此外,本试验确定了主要农艺性状及品质特征划分标准,揭示了叶色、粒色等不同类型紫苏种质在产量、α-亚麻酸含量等关键指标上的差异性规律,评价筛选的高产及特异性明显的优异种质。本研究结果为生产中应用优异基因资源及改良育种亲本提供了一定的理论依据。
In order to screen excellent Perilla frutescens germplasms, breeding parent and improve the breeding efficiency. Morphological identification, variation analysis and multivariate statistical analysis including principal components analysis and cluster analysis were carried out with 159 oil Perilla frutescens germplasms based on 11 main agronomic traits and five vital quality traits. The results showed the variations of main agronomic and quality traits ranged from 2. 60% to 35. 42%,which indicated that the tested materials were rich in genetic diversity and had good application prospect in the future. The principal components analysis showed that the six PCs,which could be regard as PBN factor（ primary branch number per plant,40. 943%）,α-LA factor（ α-linolenic acid content,21. 549%）,OA factor（ oleic acid content,12. 332%）, GP factor（ growth period,5. 810%）, SW factor（ 1000-seeds weight,4. 486%）,Y factor（ yield,4. 243%）,accounted for 89. 36% of the total variation among the germplasms. The six PCs could represent the primary information of the examined main agronomic and quality traits. Through cluster analysis,all germplasms were classified into five groups. The cluster results showed obvious specificity of different agronomic and quality traits indexes among the five groups. The study also confirmed the classification standard of main agronomic and quality traits. The variation pattern of yield and α-linolenic acid content was revealed in different types of Perilla frutescens germplasms with different leaf color and seed color. Many excellent oil Perilla frutescens germplams iwth high yield and significant specificity had be screened and evaluated in the study. The study provides a theoretical basis to breeding and applying of genetic resources.
作者
欧巧明
崔文娟
叶春雷
李进京
陈军
李忠旺
王炜
罗俊杰
OU Qiaoming;CUI Wenjuan;YE Chunlei;LI Jinjing;CHEN Jun;LI Zhongwang;WANG Wei;LUO Junjie(Biotechnology Institute,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 73007)
出处
《核农学报》
CAS
CSCD
北大核心
2018年第9期1721-1739,共19页
Journal of Nuclear Agricultural Sciences
基金
甘肃省农业科学院"现代农业生物技术集成与应用"科研创新团队(2015GAAS02)
甘肃省农业科学院农业科技创新重大专项(2016GAAS59
2017GAAS37)
国家现代农业产业体系胡麻技术体系(兰州综合试验站)(CARS-17-SYZ-6)
关键词
紫苏
种质资源
农艺性状
主成分分析
聚类分析
Perilla frutescens
germplasms
agronomic traits
principle component analysis
clustering analysis
