摘要
文章采用气相色谱-静电场轨道阱高分辨质谱(GC-Orbitrap/MS)技术,建立牛奶中30种香豆素、香兰素及其衍生物的快速筛查与定量方法。牛奶样品以乙腈为提取溶剂,超声提取,采用QuEChERS法净化,全扫描模式(Full scan)测定,基质匹配外标法定量。结果表明,30种目标物在质量浓度为2~200μg/L范围内线性关系良好,相关系数r>0.9959,检出限10~15μg/kg,定量限25~50μg/kg,平均回收率73.0%~119.1%,相对标准偏差0.5%~8.6%(n=6)。该方法前处理简单快捷、灵敏、准确,适用于牛奶中香豆素、香兰素及其衍生物的快速筛查和定量测定。
A rapid screening and quantitative analysis method for thirty coumarins and vanillins and their derivatives in milk by gas chromatography-orbitrap mass spectrometry(GC-Orbitrap/MS)was established.Milk samples were extracted with acetonitrile by ultrasonic extraction,purified by QuEChERS method,measured by Full MS mode of GC-Orbitrap/MS.The matrix matching external standard method was used for quantification.The results showed that good linearity was observed for thirty compounds in the concentration range of 2-200μg/L,with correlation coefficient(r>0.9959).The limits of detection ranged of 10-15μg/kg,and the limits of quantitation ranged from 25 to 50μg/kg.Average recoveries of the thirty compounds ranged from 73.0%to 119.1%,and the relative standard deviations(RSDs,n=6)ranged from 0.6%to 8.6%.The method was simple,quick,sensitive and accurate,suitable for the rapid screening and quantitative analysis of coumarins and vanillins and their derivatives in milk.
作者
张磊
许秀丽
沈正超
冷侯喜
梁超
裴晓燕
ZHANG Lei;XU Xiuli;SHEN Zhengchao;LENG Houxi;LIANG Chao;PEI Xiaoyan(Chinese Academy of Inspection and Quarantine,Beijing 100176,China;Guiyang Inspection Center for Food and Drug,Guiyang 550081,China;National Center of Technology Innovation for Dairy,Hohhot 010000,China)
出处
《中国乳品工业》
北大核心
2025年第4期67-73,80,共8页
China Dairy Industry
基金
国家乳业技术创新中心(2023-KFKT-23)
检科院工作经费-食品安全监管急需的化学有害物检测方法开发及监测。
