摘要
旨在比较枸杞子-菊花不同比例配伍对小鼠视网膜损伤的改善作用差异,并揭示其作用机制。采用腹腔注射碘酸钠(NaIO3)溶液建立小鼠视网膜损伤模型,设置空白组,模型组,阳性药(AREDS 2)组,枸杞子-菊花1∶1配伍低、高剂量组,枸杞子-菊花3∶1配伍低、高剂量组,枸杞子-菊花1∶3配伍低、高剂量组,造模后给药15 d,通过黑白箱检测小鼠视力;激光眼科诊断仪观察小鼠眼底情况,测量其视网膜厚度;苏木素-伊红(HE)染色法检测小鼠视网膜形态及病理变化;试剂盒检测小鼠血清及眼球组织中氧化因子水平;酶联免疫吸附测定法检测小鼠血清及眼球组织中炎症因子水平;蛋白免疫印迹法检测小鼠眼球组织中核因子E2相关因子2(Nrf2)、血红素加氧酶1(HO-1)、核因子-κB(NF-κB)蛋白表达情况。结果显示,模型小鼠给予枸杞子-菊花各比例配伍药液后,其视网膜变薄、视网膜各层排列紊乱情况得到改善,小鼠血清及眼球组织中总超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)含量升高,血清及眼球组织中丙二醛(MDA)、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-6含量降低,其中枸杞子-菊花1∶1与1∶3配伍的改善效果较好。枸杞子-菊花配伍可显著上调Nrf2、HO-1的表达水平,下调NF-κB p65的表达水平。以上结果表明,枸杞子-菊花各比例配伍均可改善小鼠视网膜损伤情况,减轻小鼠体内及眼球组织中氧化应激及炎症反应,其机制可能与调节小鼠眼球组织中Nrf2/HO-1及NF-κB信号通路相关。研究结果为枸杞子-菊花配伍临床应用于干性年龄相关性黄斑变性提供了理论依据。
This study aimed to compare the ameliorative effects of Lycii Fructus and Chrysanthemi Flos at different ratios on retinal damage in mice and to elucidate the underlying mechanisms.A retinal injury model was established by intraperitoneal injection of sodium iodate(NaIO_3)solution.The mice were divided into the following groups:blank group,model group,positive drug(AREDS 2)group,low-and high-dose groups of Lycii Fructus and Chrysanthemi Flos at 1∶1,low-and high-dose groups at 3∶1,and low-and high-dose groups at 1∶3.Administration was carried out 15 days after modeling.The visual acuity of the mice was assessed using the black-and-white box test.The fundus was observed using an optical coherence tomography device,and retinal thickness was measured.HE staining was used to observe the morphology and pathological changes of the retina.The levels of oxidative factors in serum and ocular tissues were measured using assay kits.The levels of inflammatory factors in serum and ocular tissues were detected by enzyme-linked immunosorbent assay(ELISA),and the expression of Nrf2,HO-1,and NF-κB proteins in ocular tissues was analyzed by Western blot.The results showed that after administration of Lycii Fructus and Chrysanthemi Flos at different ratios,the model group showed improved retinal thinning and disordered arrangement of retinal layers,elevated content of SOD and GSH in the serum and ocular tissues,and reduced levels of MDA,TNF-α,IL-1β,and IL-6.Lycii Fructus and Chrysanthemi Flos at 1∶1 and 1∶3 showed better improvement effects.The combination significantly upregulated the expression levels of Nrf2 and HO-1 and downregulated the expression of NF-κB p65.These results indicate that Lycii Fructus and Chrysanthemi Flos at different ratios can improve retinal damage,reduce oxidative stress,and alleviate inflammation in both the body and ocular tissues of mice.The mechanism may be related to the regulation of the Nrf2/HO-1 and NF-κB signaling pathways in ocular tissues.These findings provide a theoretical basis for the clinical application of Lycii Fructus and Chrysanthemi Flos in the treatment of dry age-related macular degeneration.
作者
李冰
郭盛
朱悦
王学森
魏丹丹
康宏杰
张文华
段金廒
LI Bing;GUO Sheng;ZHU Yue;WANG Xue-sen;WEI Dan-dan;KANG Hong-jie;ZHANG Wen-hua;DUAN Jin-ao(National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine/Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/National Administration of Traditional Chinese Medicine Key Laboratory for Chinese Medicine Resources Recycling Utilization,Nanjing University of Chinese Medicine,Nanjing 210023,China;Ningxia Innovation Center of Goji,Yinchuan 750002,China;Bairuiyuan Gouqi Co.,Lid.,Yinchuan 750200,China)
出处
《中国中药杂志》
北大核心
2025年第3期732-740,共9页
China Journal of Chinese Materia Medica
基金
国家自然科学基金区域创新发展联合基金重点项目(U21A20408)
国家自然科学基金项目(82173965)
宁夏重点研发计划项目(2021BEF01003,2021BEF02012)
江苏省重点研发计划项目(BE2021907,BE2021912)
中央本级重大增减支项目(2060302)。
关键词
枸杞子
菊花
配伍
视网膜损伤
Lyci Fructus
Chrysanthemi Flos
compatibility
retinal damage
