摘要
采用高效液相色谱（HPLC）法测定了12个菊花样品中绿原酸、黄芩苷、槲皮素、木犀草素和柯因五种活性成分的含量.色谱柱为Nov-pak C18,以0.1%磷酸-甲醇为流动相,流速0.6 mL/min,梯度洗脱,检测波长为254 nm.该方法在10-3～10-5g/mL范围内线性关系良好,保留时间和峰面积相对标准偏差（RSD）分别在0.20%～0.96%、0.23%～0.77%之间,回收率在96.5%～104.0%之间.同时建立了菊花样品的指纹图谱,采用夹角余弦和相关系数法计算了12个菊花样品的相似度,并且运用MATLAB程序对相关系数法计算值进行聚类分析,结果与实际样品种属区分一致,为菊花的质量控制和种属的区分提供依据.
A high performance liquid chromatography （HPLC） method was developed to determine chlorogenic acid, baicalin, quercetin, luteolin and chrysin in Chrysanthemum samples. The HPLC conditions were as follows： Nov-pak C18 （250×3. 9 mm i. d. , 5 μm） column, 0.1% phosphoric acid aqueous solution-methanol as mobile phase with gradient elution, flow rate with 0.6 mL/min, 254 nm as the optimum detection wavelength. Good linear relationship was established within 10^-3×10^-5 g/mL. RSDs of retention time and peak area of the five standard samples ranged from 0.20% to 0.96%, and from 0.23% to 0. 77%, respectively, showing excellent reproducibility. The recoveries were between 96.5% and 104. 0%, and the determination result was accuracy. Additionally, the fingerprints of Chrysanthemum were established with the included angle cosine and correlation coefficient methods to calculate the similarity of 12 Chrysanthemum samples. According to the result of correlation coefficient, the MATLAB procedure was applied for the cluster analysis, and the result is consistent with both the genera and the species of the actual samples. It provides the criterion for quality control and discrimination of Chrysanthemum.
出处
《分析科学学报》
CAS
CSCD
2007年第3期257-262,共6页
Journal of Analytical Science
关键词
菊花
高效液相色谱
活性成分
指纹图谱
Chrysanthemum
High Performance Liquid Chromatography （HPLC）
Fingerprints.
