摘要
目的研究美丽石斛Dendrobium formosum Roxb.ex Lindl.茎叶的化学成分及其生物活性。方法美丽石斛茎叶95%乙醇提取物采用硅胶、Sephadex LH-20及半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用PNPG法测定其α-葡萄糖苷酶抑制活性,RAW264.7模型评价其体外抗炎活性。结果从中分离得到15个化合物,分别鉴定为coniferyl p-coumarate(1)、(-)-松脂醇(2)、2,5,7-三羟基-4-甲氧基-9,10-二氢菲(3)、柚皮素(4)、spiropreussomerin A(5)、7-hydroxy-14-de-O-methyl-lasiodiplodin(6)、(4 S,5S,6Z,8E)-5-hydroxydeca-6,8-dien-4-olide(7)、(6 S,9R)-blumenol C(8)、对羟基苯甲酸(9)、间羟基苯甲酸(10)、对羟基苯丙酸(11)、5,7-二羟基-异苯并呋喃酮(12)、2-(4-羟基苯基)-乙醇(13)、β-谷甾酮(14)、β-谷甾醇(15)。化合物1、4抑制α-葡萄糖苷酶的IC 50值分别为(65.60±3.31)、(98.95±2.53)μmol/L;化合物3可抑制RAW264.7细胞产生NO,IC 50值为(3.97±0.12)μmol/L。结论化合物5~6、8、12为首次从兰科植物中分离得到,2~15为首次从该植物中分离得到。化合物1、4具有α-葡萄糖苷酶抑制活性,3具有抗炎活性。
AIM To study the chemical constituents from the stems and leaves of Dendrobium formosum Roxb.ex Lindl.and their biological activities.METHODS The 95%ethanol extract from the stems and leaves of D.formosum was isolated and purified by silica gel,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their inhibitory activities onα-glucosidase were determined by PNPG method,and their in vitro anti-inflammatory activities were evaluated by RAW264.7 model.RESULTS Fifteen compounds were isolated and identified as coniferyl p-coumarate(1),(-)-pinoresinol(2),2,5,7-trihydroxy-4-methoxy-9,10-dihydrophenanthrene(3),naringenin(4),spiropreussomerin A(5),7-hydroxy-14-de-O-methyl-lasiodiplodin(6),(4 S,5S,6Z,8E)-5-hydroxydeca-6,8-dien-4-olide(7),(6 S,9R)-blumenol C(8),p-hydroxybenzoic acid(9),m-hydroxybenzoic acid(10),p-hydroxy benzenepropanoic acid(11),5,7-dihydroxy-isobenzofuran(12),2-(4-hydroxyphenyl)-ethanol(13),β-sitostenone(14),β-sitosterol(15).The IC 50 values of compounds 1 and 4 onα-glucosidase inhibition were(65.60±3.31)and(98.95±2.53)μmol/L,respectively.Compound 3 presented inhibitory activity on NO production in RAW 264.7 cells,with IC 50 value of(3.97±0.12)μmol/L.CONCLUSION Compounds 5-6,8 and 12 are isolated from Orchidacae family for the first time,and 2-15 are first isolated from this plant.Compounds 1 and 4 haveα-glucosidase inhibitory activities,and 3 has anti-inflammatory activity.
作者
迟利航
陈惠琴
黄圣卓
吴妃
梅文莉
宋希强
戴好富
CHI Li-hang;CHEN Hui-qin;HUANG Sheng-zhuo;WU Fei;MEI Wen-li;SONG Xi-qiang;DAI Hao-fu(Key Laboratory of Genetics and Germ plasm Innovation of Tropical Special Forest Trees and Ornamental Plant,Ministry of Education,College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China;Key Laboratory of Natural Products Research and Development of Li Folk Medicine of Hainan Province,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)
出处
《中成药》
北大核心
2025年第2期467-473,共7页
Chinese Traditional Patent Medicine
基金
国家中药材产业技术体系专项项目(CARS-21)
海南省院士创新平台科研项目(YSPTZX202037)。
关键词
美丽石斛
茎叶
化学成分
分离鉴定
α-葡萄糖苷酶抑制活性
体外抗炎活性
PNPG法
RAW264.7细胞
Dendrobium formosum Roxb.ex Lindl.
stems and leaves
chemical constituents
isolation and identification
α-glucosidase inhibitory activity
in vitro anti-inflammatory activity
PNPG method
RAW264.7 cells
