摘要
目的研究荔枝核Litchi Semen盐炙品的化学成分及其抗氧化活性。方法盐炙荔枝核85%乙醇提取物采用硅胶、Sephadex LH-20、MCI、ODS、半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用DPPH、ABTS+自由基清除法评价其抗氧化活性。结果从中分离得到15个化合物,分别鉴定为去氢木香内酯(1)、菠萝香藤苷甲(2)、funingensin A(3)、(2 S)-pinocembrin-7-O-(6-O-a-L-rhamnopyranosyl-β-D-glucopyranoside)(4)、甘草素(5)、槲皮苷(6)、芦丁(7)、异鼠李素-3-O-β-芸香糖苷(8)、原花青素A2(9)、原花青素A1(10)、原儿茶酸乙酯(11)、5-羟甲基糠醛(12)、邻苯二甲酸-二(2-乙基-己基)酯(13)、烟酰胺(14)、(10 E,15Z)-9,12,13-trihydroxyoctadeca-10,15-dienoic acid(15)。化合物6~7、9~10对DPPH自由基表现出清除活性,其IC 50值分别为(12.929±1.232)、(14.104±0.946)、(10.417±1.736)、(6.944±0.030)μmol/L;化合物6~10对ABTS+自由基表现出清除活性,其IC 50值分别(21.952±0.577)、(25.683±0.625)、(22.970±1.336)、(20.210±1.435)、(18.725±0.324)μmol/L。结论化合物1、5、14~15首次从荔枝属中分离得到。化合物6~7、9~10有较强的体外抗氧化活性。
AIM To study the chemical constituents from salt-processed Litchi Semen and their antioxidant activities.METHODS The 85%ethanol extract from salt-processed Litchi Semen was isolated and purified by silica gel,Sephadex LH-20,MCI,ODS and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.DPPH and ABTS+free radical scavenging method were used to evaluate their antioxidant activities.RESULTS Fifteen compounds were isolated and identified as dehydrocostuslactone(1),ananosmoside A(2),funingensin A(3),(2 S)-pinocembrin-7-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)(4),liquiritienin(5),quercetin(6),rutin(7),isorhamnetin-3-O-β-rutinoside(8),procyanidin A2(9),procyanidin A1(10),ethyl protocatechuate(11),5-hydroxymethylfurfural(12),di(2-ethyl-hexyl)phthalate(13),nicotinamide(14),(10 E,15Z)-9,12,13-trihydroxyoctadeca-10,15-dienoic acid(15).Compounds 6-7,9-10 exhibited scavenging activities against DPPH radicals with IC 50 values of(12.929±1.232),(14.104±0.946),(10.417±1.736),(6.944±0.030)μmol/L,respectively.Compounds 6-10 exhibited scavenging activities against ABTS+radicals with IC 50 values of(21.952±0.577),(25.683±0.625),(22.970±1.336),(20.210±1.435),(18.725±0.324)μmol/L,respectively.CONCLUSION Compounds 1,5,14-15 are isolated from Litchi genus for the first time.Compounds 6-7,9-10 have strong in vitro antioxidant activities.
作者
尹蕊
杨兴
董维茂
邓徐丽
王一谋
张洪川
朱奎霖
陈章贤
何红平
董发武
YIN Rui;YANG Xing;DONG Wei-mao;DENG Xu-li;WANG Yi-mou;ZHANG Hong-chuan;ZHU Kui-lin;CHEN Zhang-xian;HE Hong-ping;DONG Fa-wu(Yunnan Key Laboratory of Southem Medicinal Utilization,College of Tradtional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China)
出处
《中成药》
北大核心
2025年第2期480-486,共7页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(31860092,82260758)
云南省高层次人才培养支持计划“青年拔尖人才”专项项目(YNWR-QNBJ-2020-255)
云南省基础研究计划面上项目(202101AT070254)。
关键词
盐炙荔枝核
化学成分
分离鉴定
抗氧化活性
DPPH自由基
ABTS+自由基
salt-processed Litchi Semen
chemical constituents
isolation and identification
antioxidant activities
DPPH free radical
ABTS+free radical
