摘要
目的 探究子痫前期患者血清CircPVT1、CircCCNB1水平及与妊娠结局的关系。方法 于2021年6月—2023年6月河北省唐山市妇幼保健院妇产科收治的子痫前期患者106例,据其妊娠结局分为妊娠不良组(n=36)和妊娠良好组(n=70)。按照子痫前期严重程度分为轻度组、重度组,分别为64例、42例。使用实时荧光定量PCR(qRT-PCR)方法测定CircPVT1、CircCCNB1的相对表达量。血清CircPVT1、CircCCNB1水平对子痫前期患者妊娠结局的预测价值绘制受试者工作特征曲线(ROC曲线)。多因素logistic回归模型分析子痫前期患者妊娠结局的影响因素。结果 与轻度组比较,重度组患者血清中CircPVT1水平显著降低,CircCCNB1水平显著升高(P<0.05)。与妊娠良好组比较,妊娠不良组患者血清中CircPVT1水平显著降低、CircCCNB1水平显著升高(P<0.05)。与血清CircPVT1、CircCCNB1单独预测比较,二者联合预测子痫前期患者妊娠结局的ROC曲线下面积(AUC)显著升高(Z=2.883,P=0.006;Z=3.772,P<0.001)。收缩压、尿酸(UA)、CircPVT1、CircCCNB1均为影响子痫前期患者妊娠不良结局的危险因素(P<0.05)。结论 子痫前期不良妊娠结局患者血清CircPVT1水平降低、CircCCNB1水平升高,对妊娠结局有一定的预测价值。
Objective To investigate the relationship between serum CircPVT1 and CircCCNB1 levels and pregnancy outcome in patients with preeclampsia.Methods A total of 106 patients with preeclampsia admitted to the Department of Obstetrics and Gynecology of our hospital from June 2021 to June 2023 were divided into the adverse pregnancy group(n=36)and the good pregnancy group(n=70)according to their pregnancy outcomes.According to the severity of pre-epilepsy,64 cases were divided into mild group,and the other 42 cases were divided into severe group.The relative expression levels of CircPVT1 and CircCCNB1 were determined by real-time quantitative fluorescence PCR(qRT-PCR).The predictive value of serum CircPVT1 and CircCCNB1 levels in pregnancy outcomes of patients with preeclampsia was plotted by ROC curve.Multivariate logistic regression model was applied to analyze the influencing factors of pregnancy outcomes in patients with preeclampsia.Results Compared with the mild group,the serum CircPVT1 level in severe group was significantly decreased,and the CircCCNB1 level was significantly increased(P<0.05).Compared with the good pregnancy group,the serum CircPVT1 level in poor pregnancy group was significantly decreased and the CircCCNB1 level was significantly increased(P<0.05).Compared with the independent prediction of serum CircPVT1 and CircCCNB1,the combined prediction of the two had a obviously higher AUC of pregnancy outcomes in preeclampsia patients(Z=2.883,P=0.006;Z=3.772,P<0.001).Systolic blood pressure,UA,CircPVT1 and CircCCNB1 were all risk factors for adverse pregnancy outcomes in preeclampsia patients(P<0.05).Conclusion Serum CircPVT1 level decreased and CircCCNB1 level increased in patients with adverse pregnancy outcome of preeclampsia,which has certain predictive value for pregnancy outcome.
作者
宁书芬
孙莉莉
唐显赫
NING Shufen;SUN Lili;TANG Xianhe(Department of Obstetrics,Tangshan Maternal and Child Health Hospital,Tangshan 063000,China)
出处
《中国煤炭工业医学杂志》
2024年第6期575-580,共6页
Chinese Journal of Coal Industry Medicine
基金
河北省2024年度医学科学研究课题计划(编号:20241788)
