摘要
目的:检测子痫前期孕妇胎盘组织及血清中干扰素-γ诱导蛋白10(IP-10)的表达情况,探讨其与子痫前期发病的关系。方法:收集正常妊娠、轻度子痫前期、重度子痫前期孕妇的胎盘组织及血清,采用ELISA、实时荧光定量PCR及Western blot方法检测IP-10在3组孕妇胎盘组织及血清中的表达变化。结果:ELISA结果显示,与正常对照组相比,轻度子痫前期组、重度子痫前期组孕妇血清中IP-10表达均明显增加(P均<0.05);实时荧光定量PCR及Western blot结果显示,与正常对照组相比,轻度子痫前期组、重度子痫前期组孕妇胎盘组织中IP-10 mRNA及蛋白表达水平均有不同程度增高(P均<0.05)。结论:子痫前期孕妇胎盘及血清中IP-10表达升高,可能与子痫前期的发病有关。
Objective：To detect interferon-gamma inducible protein 10（IP-10） in the placenta and serum of gravidas with preeclampsia in order to evaluate the relationship of IP-10 with preeclampsia.Methods：We collected placenta and serum from normal gravidas,gravidas with mild preeclampsia and gravidas with severe preeclampsia.The expression of IP-10 in the placenta and serum was detected by ELISA,real-time PCR and Western blot.Results：As the results of ELISA,the IP-10 expression levels in serum of patients with severe or mild preeclampsia were significantly higher than those in normal pregnancy group（P0.05）.And as the results of real-time PCR and Western blot,the expression levels of IP-10 in the placenta with severe or mild preeclampsia were all significantly higher than those in normal pregnancy group（P0.05）.Conclusion：The elevated IP-10 expression in the placenta and serum may play an important role in preeclampsia.
出处
《现代妇产科进展》
CSCD
2012年第9期669-672,共4页
Progress in Obstetrics and Gynecology
基金
南通市社会发展计划(No:S2008032)
江苏省卫生厅"科教兴卫工程"苏州大学附属第一医院临床免疫实验室开放课题(No:KF200973)
