摘要
目的了解南京市分离鉴定的人源布鲁氏菌种型、生物型及基因型分布概况。方法收集2017-2022年南京市3家哨点医院微生物实验室分离鉴定的89株人源布鲁氏菌,通过生物学方法及布鲁氏菌核酸检测(BCSP31-PCR和AMOS-PCR)进行种型鉴定;结合A、M因子血清学检测结果,对鉴定为羊种布鲁氏菌的分离株进一步进行生物分型。同时,运用多位点可变数目串联重复序列分析(multiple-locus variable number tandem repeat analysis,MLVA)、多位点序列分型(multilocus sequence typing,MLST)及单核苷酸多态性(single nucleotide polymorphism,SNP)方法进行基因型分析。结果2017-2022年,南京市分离鉴定的89株布鲁氏菌均为羊种布鲁氏菌。其中,羊种布鲁氏菌生物3型占82.02%(73/89),生物1型占17.98%(16/89)。MLVA分型发现,89株羊种布鲁氏菌均属于"东地中海"簇,可分为50个MLVA基因型。其中panel 1有3个基因型,分别为42型(84.27%,75/89)、63型(8.99%,8/89)、43型(6.74%,6/89)。MLST-9和MLST-21结果均为ST8型,核心基因组多位点序列分型(core genome multilocus sequence typing,cgMLST)将89株菌株分为11个基因型。SNP分析共发现4013个SNPs位点,各菌株间SNPs范围为0~409个,涉及59个SNPs基因型。结论南京市分离鉴定的人源布鲁氏菌菌株全部为羊种布鲁氏菌,以生物3型为主。MLVA聚类为"东地中海"簇。传统MLST-9和MLST-21结果全部为ST8型,而cgMLST将所有菌株分为11个基因型,具有更高的分辨力。
Objective To study the distribution of species type,biotype and genotype of human Brucella isolated and identified in Nanjing.Methods A total of 89 strains of human Brucella were collected from microbiology laboratories of three sentinel hospitals in Nanjing from 2017 to 2022.The species type was identified using biological methods and Brucella nucleic acid detection(BCSP31-PCR and AMOS-PCR).Further biotyping of Brucella melitensis isolates was conducted by serological results of A and M factors.Meanwhile,genotype analysis was performed using multiple-locus variable number tandem repeat analysis(MLVA),multilocus sequence typing(MLST)and single nucleotide polymorphism(SNP).Results From 2017 to 2022,89 strains of Brucella isolated and identified in Nanjing were all Brucella melitensis.Among them,Brucella melitensis biotype 3 accounted for 82.02%(73/89),and biotype 1 accounted for 17.98%(16/89).MLVA typing showed that 89 strains of Brucella melitensis belong to the"Eastern Mediterranean"cluster and could be divided into 50 MLVA genotypes;among which panel 1 had 3 genotypes,namely Type 42(84.27%,75/89),Type 63(8.99%,8/89)and Type 43(6.74%,6/89).The results of MLST-9 and MLST-21 were both ST8,and the core genome multilocus sequence typing(cgMLST)classified 89 strains into 11 genotypes.SNP analysis revealed a total of 4013 SNP loci,with SNPs ranging from 0 to 409 across different strains,involving 59 SNP genotypes.Conclusions The human Brucella strains isolated and identified in Nanjing are all Brucella melitensis,mainly biotype 3.The MLVA cluster is the"Eastern Mediterranean"cluster.The traditional MLST-9 and MLST-21 typing results are all ST8 type,while cgMLST divides all the strains into 11 genotypes with higher resolution.
作者
王炜翔
周璐
苏晶晶
张楠
洪捷
周伟忠
鲍倡俊
谈忠鸣
Wang Weixiang;Zhou Lu;Su Jingjing;Zhang Nan;Hong Jie;Zhou Weizhong;Bao Changjun;Tan Zhongming(Department for Acute Infectious Disease Control,Nanjing Center for Disease Control and Prevention,Nanjing 210000,China;Jiangsu Provincial Center for Disease Control and Prevention,NHC Key Laboratory of Enteric Pathogenic Microbiology,Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases,Nanjing 210009,China)
出处
《中华地方病学杂志》
北大核心
2024年第10期775-782,共8页
Chinese Journal of Endemiology
基金
江苏省卫生健康委科研重点项目(ZD2021037)
