摘要
本文应用聚丙烯酰胺凝胶圆盘电泳分析比较我国七个流行区(贵州独山、贵州荔波、四川乐山、安徽泾县、浙江安吉、湖北谷城和福建建阳)周期型马来丝虫微丝蚴的七种酶同工酶型。结果显示,七个地区的磷酸葡糖异构酶(GPI)、葡糖-6-磷酸脱氢酶(G6PD)、乳酸脱氢酶(LDH)和过氧化物酶(PO)的同工酶型没有任何差异。但是上述地区的苹果酸脱氢酶(MDH)和磷酸葡糖变位酶(PGM)各出现两种同工酶型,即贵州省内的两个地区的不同于另外五个地区的结果。酯酶染色没有区带出现。
The isoenzyme patterns of seven enzymes of microfilaria periodic Brugiamalayi from seven endemic areas (Dushan Guizhou, Libo Guizhou,Leshan Sichuan, Jingxian Anhui, Anji Zeejiang, Gucheng Hubei andJianyang Fujian) were comparatively analysed by disc electrophoresis.It was shown that there existed no difference in isoenzyme patterns ofGPI, G6PD, LDH and PO among microfilariae of seven areas. However,we still found that there existed obvious difference in isoenzyme patternsof MDH and PGM between the two areas of Guizhou and the other fiveareas. No band was found in the isoenzyme pattern of EST.
关键词
马来丝虫
微丝蚴
圆盘电泳
同功酶
Brugia malayi
microfilaria
disc electrophoresis
isoenzyme pattern
