摘要
在含NaCl的选择培养基中获得的大豆小真叶愈伤组织及再生植株的过氧化物酶活性增高,蛋白质含量降低,愈伤组织中的脯氨酸含量随NaCl浓度增高而急剧上升。在盐胁迫下过氧化物酶活性和脯氨酸含量增高,蛋白质含量降低是对盐胁迫的生理适应。
The young true leaves of soybean were cultured in the several concentration NaCl medium. After cultured for 20-24 days, the peroxidase active, protien and proline content of callus tissues and 2 - 4 cm high regenerated plantlet were measured. The results showed that peroxidase activity of callus tissues and regenerated plantlet under salt stress were higher than control. Except NaCl 0. 25% , peroxidase activeity of callus tissues and regenerated plantlet increased with the increase of NaCl concentration. But under several salt stress and control, the peroxidase active of regenerated plantlet was higher than that of callus tissues. Protein content of regenerated plantlet was higher than callus tissues under several NaCl concentration and control. Protein content of callus tissues and regenerated plantlet under salt stress was lower than control, and decreased with the increase NaCl concentration, except NaCl 0. 4%. The accumulation of proline was higher in callus tissues than in control and rapidly increased with increase of NaCl concentration. The proline accumulation was in relation to the decrease of protein content in callus tissues. Under salt stess, the increase of peroxidase activity, decrease of protein contect in callus tissues and regenerated plantlet and prolline accumulation in callus tissues, were a physiological adaptation to salt stress.
出处
《大豆科学》
CAS
CSCD
北大核心
1992年第1期70-73,共4页
Soybean Science
基金
山西省自然科学基金
