摘要
观察了 5 0Hz,0 .2mT或 6 .0mT的电磁场暴露对大豆的萌发过程、丙二醛 (MDA)含量 ,超氧化物歧化酶(SOD)、过氧化氢酶 (CAT)、过氧化物酶 (POD)、蛋白酶活性和可溶性蛋白含量以及幼苗根茎的组织形态学影响 .结果表明 :电磁场暴露下 ,大豆的开始萌发时间由 32h提前到 8h ,萌发率到达 5 0 %的时间由 72h分别提前到 4 8h(0 .2mT组 )和 4 0h(6 .0mT组 ) ;MDA含量随磁场强度的增大而下降 ;SOD ,POD活性随磁场强度的增大而升高 ,而CAT活性在 0 .2mT暴露组下降 ,在 6 .0mT暴露组升高 ;蛋白酶活性和可溶性蛋白含量分别随磁场强度的增大升高或降低 .幼苗根茎细胞的大小和形态没有明显变化 .结果提示 ,极低频电磁场可以改变抗氧化酶的活性 ,减缓脂质过氧化 ,增强蛋白酶的活性 ,促进大豆的萌发过程 .图 4参
Soybean seeds during germination were exposed to a sinusoidal 0.2 mT or 6.0 mT electromagnetic field(EMF) at 50 Hz. The effects of EMFs on germination ratio, malonaldehyde (MDA) content, the activities of superoxide dismutase (SOD), catalase (CAT),peroxide dismutase (POD) and proteinase, soluble protein content in soybean seeds, and the morphological structure of soybean seedlings were measured. The results indicated that the time germination started and germination percentage got to 50% was earlier for the seeds exposed to EMFs than for the control. MDA content decreased and the activities of SOD and POD increased with the increase of magnetic field intensity. The activities of CAT decreased in 0.2 mT field but increased in 6.0 mT field. Proteinase activity increased and soluble protein content decreased with the increase of magnetic field intensity. No significant difference induced by EMFs was observed in the morphological structure of soybean seedlings. These results suggested that 50 Hz EMFs could increase the activities of anti oxidation enzymes, alleviate lipid per oxidation, enhance proteinase activity and therefore accelerate the germination of soybean seeds. Fig 4, Ref 6
出处
《应用与环境生物学报》
CAS
CSCD
2002年第5期482-484,共3页
Chinese Journal of Applied and Environmental Biology
基金
上海市重点学科资助~~
关键词
极低频电磁场
萌发期
大豆
生物学效应
抗氧化酶
蛋白酶
可溶性蛋白
extremely low frequency electromagnetic fields
germination
antioxidant enzyme
proteinase
soluble protein
soybean
