摘要
目的 :观察大鼠AD模型颞叶和额叶在 98dB宽频噪音暴露 5min前后不同脑区ERK、GDNF表达及ABR阈值的影响。方法 :取体重 15 0～ 2 2 0 gSD大鼠 ,雌雄不拘 ,经行为训练筛选 ,剔除记忆差 (指“稍有记忆”和“无记忆”)大鼠后 ,随机分 3组 :双侧海马CA1区 (AP3.2～ 3.4 ,L2 .0～ 2 .4 ,H2 .8～ 3.0 )微量注射谷氨酸组 (n =8) ;双侧海马CA1区微量注射生理盐水组 (n =8) ;空白对照组 (n =10 )。采用WesternBlot及图像分析技术 ,结合ABR测定方法。结果 :①空白对照组大鼠额叶ERK表达明显多于其它两组鼠 ,且加噪音后有较显著的上调趋势 ;②各组动物颞区皮质神经元加噪音后ERK表达明显增强 ,且均强于额叶各组 ,也有较显著的上调趋势 ;③空白对照组大鼠额叶GDNF加噪音后表达多于加噪音前的同组鼠 ,有较明显的上调趋势 ;④谷氨酸组加噪音后颞区皮质神经元GDNF表达有较明显的下调趋势 ;⑤颞区空白对照组GDNF表达远弱于额叶。结论 :AD模型大鼠额叶ERK表达较少 ,不受噪音刺激影响 ,颞区则相反 ,且明显上调 ;宽频噪音抑制大鼠AD模型颞叶GDNF表达。
Aim:To investigate the change of ERK,GDNF expression activity in temple and frontal lobe of AD rat after 96 dB wide band noise exposure. Methods:Experimental group SD rats(weight from 150～220 g),either male or female, were randomly assigned to three groups:control( n =10);physiological brine injected control( n =8);glutamic acid injected group( n =8) after getting rid of memory loss(indicating 'a little memory' and 'no memory')rats culled by behavior training.The rats were micro injected at the sterotaxic device(AP3.2～3.4,L2.0～2.4,H2.8～3.0)by glutamic acid or same volume of physiological brine in the each side of hippocampus CA1.Western Blot and image quantitative analysis technique, combined with auditory brainstem response (ABR) measurement. Results:①Expressions of ERK in frontal cortex for control rat are much more than that of other group, which has a obvious up regulation after being given 96 dB noise.②Expression of ERK in temple cortex for 3 groups rat have a increase(plus noise),moreover, they are stronger than expression in frontal cortex for different group. ③GDNF in frontal cortex for control rat have a higher expression than that of the same group before adding noise (up regulation).④GDNF expression in temple lobe for glutamatic acid group have a remarkable down regulation trend.⑤Expression of GDNF in frontal for control rat is much less than in temple. Conclusion: ERK in frontal cortex for AD model rat have fewer expressions and not being affected by 96 dB noise, but it is reverse in temple.Wide band frequency noise can reduce the expression of GDNF in temple lobe of AD model rat.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2002年第4期324-328,共5页
Chinese Journal of Applied Physiology
关键词
宽频噪音
大鼠
AD模型
脑区
ERK
GDNF
表达
ABR阈值
extracellular regulated protein kinases(ERK)
wide band noise
glial derived neurotrophic factor(GDNF)
