摘要
目的 观察衰老与阿尔采默病AD模型大鼠颞叶和颞叶在 96dB宽频噪音暴露下NOS1表达及ABR阈值的影响。方法 采用WesternBlot及图像分析技术 ,结合ABR测定方法。结果 双侧海马CA1 区微量注射谷氨酸前 ,电击后 2 4h有记忆者为 69 6 % ,损伤后仅为 1 4 7% ,两者差别有非常显著性 (P <0 0 1 )。 3组大鼠加量噪音自身前后ABR阈值差值都有显著性差异 (P <0 0 1 )。谷氨酸组在加噪音前ABR阈值与对照组有差异 (P <0 0 5)。谷氨酸组大鼠颞叶、颞叶NOS1表达多于加噪音后的同组鼠 ,有较明显的下调趋势。结论 宽频噪音抑制AD模型大鼠颞叶。
Objective To investigate the change of nitric oxide synthase one(NOS1) activity in temple and frontal lobe of AD rat after 96 dB wide frequency noise exposure.Methods Western Blot and image quantitative analysis technique,combined with auditory brainstem response (ABR) measurement.Results Before injection of glutamatic acid in two sides of hippocampus CA 1,memoried rats are 69.6% after 24 hours by electric stimulation,but is only 14.7% after injury and differences are very notable ( P <0.01).Contrast of ABR threshold from beginning to end in three group of rats is very marked( P <0.01).ABR threshold contrasted with two group control,that of rat in glutamatic acid group before being given noise stimulation are remarkable ( P <0.05).expression of NOS1 in temple and frontal lobe of glutamatic acid group are much more than those of themselves after being given noise stimulation.Conclusion Wide band frequency noise can reduce the expression of NOS1 in temple and frontal lobe of AD model rat.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第6期701-703,共3页
Chinese Journal of Public Health
