摘要
目的:观察脑内远位触液神经元内p-p38丝裂原活化蛋白激酶(MAPK)的分布及其在噪声应激时的表达。方法:用霍乱毒素亚单位B与辣根过氧化物酶复合物(CB-HRP)标记和免疫组织化学相结合的双重标记技术,观察SD大鼠脑实质内远位触液神经元中p-p38MAPK的分布。进一步制作噪声应激动物模型,观察噪声应激后该类神经元中p-p38MAPK的表达变化。结果:在脑干的特定部位恒定出现被CB-HRP标记的两组神经细胞簇,其他脑区未见CB-HRP标记神经细胞簇。不予应激刺激,该细胞簇内仅有个别神经元见有CB-HRP/p-p38MAPK;噪声应激刺激1d时,上述特定部位细胞簇的CB-HRP/p-p38MAPK双重标记神经元数目没有明显变化;噪音应激刺激5d时,CB-HRP/p-p38MAPK双重标记神经元数目较对照组显著增多(P<0.05);噪音应激刺激10d时CB-HRP/p-p38MAPK双重标记神经元数目较对照组显著增多(P<0.05);噪音应激刺激20d时,CB-HRP/p-p38MAPK双重标记神经元数目较对照组显著增多(P<0.01)。结论:在脑干特定部位恒定存在的两组被CB-HRP标记的细胞团为远位触液神经元,其中少数触液神经元有p-p38MAPK表达,且当给予动物噪声应激刺激时,p-p38MAPK免疫阳性神经元和CB-HRP/p-p38MAPK双重标记神经元数量显著增加,提示脑实质内的这种远位触液神经元中的p-p38MAPK可能参与了机体对噪声应激的信息传递或调控,其作用随应激天数增加而日趋增强。
To observe the distribution and expression of p-p38MAPK in the distal cerebrospinal fluid contacting neurons in brain of rat by noise stress.Methyls： By a doubl-labelled method combing the tracing of CB-HRP and the immuhistochemical technique p- p38MAPK, the distribution and expression of p-p38MAPK in the distal cerebrospinal fluid contacting neurons（csf-cn） were observed following noise stress. Expression of p-p38MAPK and double-labelled of CB-HRP/p-p38MAPK were also observed in rat brain after noise stress. Results： Two groups of CB-HRP labeled neuron clusters consistently appeared in certain regions of the brainstem but none in other regions of the brain. Without noise stress exposure, only a few neurons were found double-labeled by CB-HRP/p-p38MAPK. After 1 day noise stress exposure, only few neurons double-labeled by CB-HRP/p-p38MAPK were observed in the above-mentioned regions. After 5 days, the number of neurons double-labeled by CB-HRP/p-p38MAPK increased significantly compared with the control group （P〈0.05）. After 10 days, the number of neurons double-labeled by CB-HRP/p-p38MAPK increased significantly compared with the control group （P〈0.05）. After 20 days, both of the numbers of neurons double-labeled by CB-HRP/p-p38MAPK increased significantly compared with that of the control group （P 〈 0.01 ）. Conclution： Two groups of distal cerebrospinal fluid con- tacting neuron clusters consistently existed in certain regions of the brain parenchyma, and in these clusters only a few neurons conrained p-p38MAPK. After noise stress exposure of different durations （days 1, 5, 10, 20）, the number of distal cerebrospinal fluid contacting neurons with p-p38MAPK increased significantly with increasing days. The results indicate that distal cerebrospinal fluid contacting neurons are special neurons existing consistently in brain, including distal cerebrospinal fluid contacting neurons with p- p38MAPK which may participate in the whole procedure of signal transduction or central modulation in noise stress response and play greater roles with increasing days.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2007年第4期419-423,I0009,共6页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助项目(30570974)
江苏省自然科学基金资助项目(BK2004028)
江苏高校自然科学基金资助项目(04KJB320147)
