摘要
目的 :观察单一冷应激对大鼠脾脏NK细胞活性的影响以及脑内Fos表达。方法 :大鼠置于 4℃的冷室 4h。采用YAC 1细胞51Cr释放法测定脾脏NK细胞的活性。用免疫细胞化学观察脑内有关核团的Fos表达 ,以及PVN和LC中Fos表达阳性的神经元的性质。结果 :单一冷应激能使脾脏NK细胞活性明显下降 ,下丘脑室旁核 (PVN)和脑干蓝斑 (LC)出现明显的Fos表达。双染实验表明 ,PVN中有部分神经元同时表达精氨酸加压素 (AVP) ,LC中大多数神经元同时表达酪氨酸羟化酶 (TH)。结论
Aim:To observe the effect of cold stress on the splenic NK cell activity and the c fos expression in rat brain.Methods:Rats were maintained in cold chamber at 4℃ for 4 h. The 51 Cr release assay from YAC 1 cells was used to determine the splenic NK cell activity and the double staining of ABC method was employed to observe the immunoreactive expression of Fos, arginine vasopressin and tyrosine hydroxylase. Results:Cold stress could induce a marked suppression of splenic NK cell activity and a significant expression of Fos protein in hypothalamic paraventricular nucleus(PVN), as well as in the brainstem locus coeruleus(LC). Double staining showed co expression of Fos and arginine vasopressin in some of PVN neurons, and co expression of Fos and tyrosine hydroxylase in the majority of LC neurons. Conclusion:The vasopressinergic neurons in PVN and the catecholaminergic neurons in LC might be probably involved in the suppression of splenic NK cell activity induced by cold stress.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2002年第4期313-316,共4页
Chinese Journal of Applied Physiology
基金
中日校际合作研究基金资助 (EE13 40 0 5 )
