摘要
选用清洁级雄性昆明小鼠30只,随机分均为冷应激组、冷应激+VC组和对照组,冷应激和冷应激+VC组每天在0℃下给2h的冷刺激,冷应激+VC组补充300μg/g的VC,试验期1周。结果,在冷应激环境中,小鼠早晚体质量均表现明显下降趋势,胸腺、脾脏和肺脏的重量均显著降低(P<0.05),器官指数有所下降,但差异不显著(P>0.05);胸腺小体数量增加,胸腺细胞数量明显减少,动脉周围淋巴鞘变薄,肺泡腔变大,肺泡壁变薄,肺泡间毛细血管减少。补充VC后,小鼠早晚体质量下降趋势减缓,肺脏重量和器官指数显著增加(P<0.05),胸腺和脾脏重量及器官指数也有所增加,但差异不显著(P>0.05)。胸腺小体体积减小,淋巴细胞数量有所增加,脾小结数量以及体积都有所增加,动脉周围淋巴鞘增厚,肺泡壁明显增厚,肺泡间结缔组织增加,毛细血管增多。结果表明补充VC可以改善冷应激对免疫器官和呼吸器官造成的不良影响。
The 30 clean level Kunming male mice were randomly divided into cold stress group, cold stress ＋Vc group and control group （n= 10）. The cold stress and cold stress ＋ Vc group were given 2 h cold stimulation at 0 ℃ ,and the cold stress＋Vc group were also given Vc of 300 μg/g BW, the experiment lasted for a week. In cold stress environment, the body weight of mice had an obvious decrease trend; the weight of thymus,spleen and lung were significant decreased （P〈0. 05） ,and the organ indexes were also decreased, but no significant dfference were found （P 〉0.05） ; the quantity of thymic corpuscle was increased, thymocyte cells were obvious decreased, periarterial lymphatic sheaths became thin, alveolar cavity became larger, alveolar wall became thinner,alveolar capillaries were decreased. In complement Vc group,the decrease trend of body weight in mice obviously slew down; the weight and index of lung were significangtly increased （P〈0.0.05） ,and the weight and index of thymus and spleen were slightly increased （P〉0.05）; the volume of thymic corpuscle was decreased,and thymocyte cells were increased, the quantity and volume of splenic nodule were increased,the periarterial lymphatic sheaths became thick,the alveolar wall was obviously thicked, the alveolar capillaries and connective tissue were increased. Above results showed supplement of Vc can attenuat the adverse effect of cold stress on immune and respiratory organs.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第11期1738-1743,共6页
Chinese Journal of Veterinary Science
基金
教育部科学技术研究重点项目(210095)
安徽省自然科学基金资助项目(090411001
1208085QC68)
安徽科技学院重点学科基金项目(AKXK20101-2)
关键词
维生素C
冷应激
免疫器官
呼吸器官
组织结构
vitamin c
cold stress
immune organ
respiratory organ
tissues structure
mouse
