摘要
胶体金免疫层析法具有高特异性、高灵敏度、高重复性等特点,能够准确地检测出样品中的目标物,在快速检测领域具有广泛应用。本研究将胶体金免疫层析法用于快速检测动物源食品(猪肉、鸡肉、鱼肉、虾肉、鸡蛋)中的诺氟沙星(Norfloxacin,NOR)残留,对方法进行验证,相对准确度为97.0%。方法的检出限为2μg/kg,检测性能强。在上述5种不同基质样品中,NOR胶体金快速检测方法的灵敏度为99%、特异度为98%、假阳性率为2%、假阴性率为1%。该方法简单快速、灵敏准确,适于复杂基质中NOR的快速精准检测。
Colloidal gold immunochromatography has the characteristics of high specificity,high sensitivity,and high repeatability,which can accurately detect the targeted analytes in the samples,and has a wide range of applications in the field of rapid detection.This study used colloidal gold immunochromatography to rapidly detect norfloxacin(NOR)residues in animal-derived foods(pork,chicken,fish,shrimp,and eggs)and validated the method with a relative accuracy of 97.0%.The limit of detection was down to 2μg/kg,showing high detectability.By testing the above-mentioned five different matrix samples,the NOR colloidal gold rapid detection method attained a sensitivity of 99%,specificity of 98%,false positive rate of 2%,and false negative rate of 1%.This method proved simple,rapid,sensitive,and accurate,and thereby was practically applicable for the rapid,precise detection of NOR in complicated matrices.
作者
陈文硕
温宇浩
娄婷婷
潘红蕊
李金花
CHEN Wen-shuo;WEN Yu-hao;LOU Ting-ting;PAN Hong-rui;LI Jin-hua(Industrial Product Safety Technology Center,Tianjin Customs,Tianjin 300457,China;Shandong Key Laboratory of Coastal Environmental Processes,Coastal Zone Ecological Environment Monitoring Technology and Equipment Shandong Engineering Research Center,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;School of Bioengineering,Tianjin University of Science and Technology,Tianjin 300222,China)
出处
《化学试剂》
CAS
2025年第1期92-96,共5页
Chemical Reagents
基金
海关总署课题项目(2023HK084)
天津市科技支撑重点项目(20YFZCSN00630)
国家自然科学基金项目(22176210)
山东省自然科学基金项目(ZR2020KC032)。
关键词
胶体金免疫层析法
诺氟沙星
方法验证
动物源食品
快速检测
colloidal gold immunochromatography
norfloxacin
method validation
animal-derived foods
rapid detection
