摘要
为获得云芝菌丝体最佳产香培养条件和其挥发性成分,采用单因素试验法考察了不同碳源、氮源、pH值、培养基体积、培养温度、培养时间对云芝菌丝体产生挥发性成分的影响,通过响应面法优化其培养条件,并结合气相色谱-质谱联用法对云芝菌丝体挥发性成分进行分析。结果表明:培养基体积、培养时间、培养温度对云芝菌丝体挥发性成分具有显著影响,最佳培养条件为以蔗糖为碳源、不添加氮源、培养基体积120 mL、培养温度27.5℃、培养时间27 d,在此条件下验证试验结果与理论预测的误差为0.315%。从云芝菌丝体中共检测出6种挥发性成分,包括脂肪醇类3种、芳香族类1种、倍半萜烯类2种,其中1-表双环倍半水芹烯和(E)-菖蒲烯为主要成分,相对含量分别为29.96%和29.17%,为云芝菌丝体在微生物香料的进一步开发应用提供科学依据。
In order to obtain the optimal culture conditions and volatile components of Trametes versicolor mycelium,a single factor experimental method was used to study the effects of different carbon sources,nitrogen sources,pH values,medium volume,culture temperature,and culture time on the production of volatile compon-ents in T.versicolor mycelium.The culture conditions were further optimized by response surface methodology and the volatile components of T.versicolor mycelium were analyzed by gas chromatography-mass spectrometry.The results showed that the volume of culture medium,culture time,and culture temperature had a significant im-pact on the volatile components of T.versicolor mycelium.The optimal culture conditions were sucrose as the carbon source,no nitrogen source added,120 mL of culture medium volume,27.5℃ of culture temperature,and 27 days of culture time.Under these conditions,the error between the validation test results with the theoretical prediction is 0.315%.A total of 6 volatile components were detected from the mycelium of T.versicolor,includ-ing 3 alcohols,1 aromatics,and 2 sesquiterpenes.Among them,1-epibicyclosesquiphellandrene and(E)-cala-menene were the main components,with relative contents of 29.96% and 29.17%,respectively.This provides a basis for the further development and application of T.versicolor mycelium in microbial fragrances.
作者
吴博宇
鲁斌
尹文行
萧卫泓
王玮
严冬
赵平
Wu Boyu;Lu Bin;Yin Wenhang;Xiao Weihong;Wang Wei;Yan Dong;Zhao Ping(Key Laboratory of National Forestry and Grassland Administration on Highly-efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650233,China;Key Laboratory of Ministry of Education for Forest Resources Conserva-tion and Utilization in the Southwest Mountains of China,Southwest Forestry University,Kunming Yunnan 650233,China;Lushui Sub-bureau of Gaoligongshan National Nature Reserve,Nujiang Yunnan 673200,China)
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2024年第5期207-214,共8页
Journal of Southwest Forestry University：Natural Sciences
基金
国家自然科学基金项目(32060107,32060327)资助。
