摘要
研究猪感染口蹄疫病毒(Foot-and-mouth disease virus,FMDV)后程序性死亡受体-1(Programmed death-1,PD-1)及其配体(PD-L1、PD-L2),细胞因子[IL-2(Interleukin-2)、IL-10(Interleukin-10)、IFN-γ(Interferon-γ)]mRNA转录水平和外周血单个核细胞(Peripheral blood mononuclear cells,PBMCs)增殖能力,探讨PD-1/PD-Ls通路在FMDV引起免疫抑制过程中的作用机制。结果表明,猪感染FMDV后3、7、14、17 d,PD-1的mRNA转录水平显著或极显著升高,感染后7 d达到峰值,之后逐渐降低;感染FMDV后3 d和7 d,PD-L1的mRNA转录水平极显著升高;感染FMDV后3 d,PD-L2的mRNA转录水平显著升高。感染FMDV后3、7 d,IL-2的mRNA转录水平显著下降;感染FMDV后1、3 d,IFN-γ的mRNA转录水平极显著下降,IL-2和IFN-γ的mRNA转录水平与PD-1及其配体mRNA转录水平变化趋势相反;感染FMDV后3、7、14 d,IL-10的mRNA转录水平显著或极显著升高,与PD-1及其配体mRNA转录水平变化趋势相同。感染FMDV后,全血中FMDV的病毒载量随着感染后时间的推进逐渐升高,感染后7 d达到峰值,与PD-1及其配体mRNA转录水平变化趋势总体上相同。感染后7 d,PBMCs的增殖能力明显降低。综上,FMDV感染猪后,总体上上调了PD-1及其配体的mRNA转录水平,是FMDV感染引起机体免疫抑制的重要致病机制。
This study investigated the mRNA transcription levels of programmed death-1(PD-1)and its ligands(PD-L1,PD-L2),cytokines[interleukin-2(IL-2),interleukin-10(IL-10),interferon-γ(IFN-γ)],and proliferation ability of peripheral blood mononuclear cells(PBMCs)in pigs infected with foot and mouth disease virus(FMDV),to explore the mechanism of the PD-1/PD-Ls pathway in the immunosuppressive process induced by FMDV.The results showed that at 3,7,14 and 17 days after infection with FMDV,the transcription level of PD-1 mRNA significantly or extremely significantly increased,reaching a peak at 7 days after infection,and then gradually decreased.At 3 and 7 days after infection with FMDV,the transcription level of PD-L1 mRNA extremely significantly increased.At 3 days after infection with FMDV,the transcription level of PD-L2 mRNA significantly increased.At 3 and 7 days after infection with FMDV,the transcription level of IL-2 mRNA significantly decreased.At 1 and 3 days after infection with FMDV,the transcription levels of IFN-γmRNA extremely significantly decreased.The change trend of transcription levels of IL-2 and IFN-γmRNA showed opposite to those of PD-1 and its ligands mRNA.At 3,7 and 14 days after infection with FMDV,the transcription level of IL-10 mRNA significantly or extremely significantly increased,which was consistent with the change trend of the transcription levels of PD-1 and its ligands mRNA.After infection with FMDV,the viral load of FMDV in the whole blood gradually increased with the progression of infection time,reaching a peak at 7 days after infection,which was generally consistent with the change trend of PD-1 and its ligand mRNA transcription levels.At 7 days after infection,the proliferation ability of PBMCs obviously decreased.Overall,after FMDV infection,the transcription levels of PD-1 and its ligands mRNA are upregulated,which is an important pathogenic mechanism for immune suppression caused by FMDV infection.
作者
岳锋
周娟娟
朱艳平
郭东光
夏黎明
祝仰钊
王选年
YUE Feng;ZHOU Juanjuan;ZHU Yanping;GUO Dongguang;XIA Liming;ZHU Yangzhao;WANG Xuannian(School of Biological Engineering,Xinxiang University,Xinxiang 453003,China;Xinxiang Zoonosis Key Laboratory,Xinxiang 453003,China;College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China)
出处
《河南农业科学》
北大核心
2024年第8期126-132,共7页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金项目(31702216)
河南省科技攻关项目(242102110022)
河南省重点研发专项(241111112900)
河南省高等学校青年骨干教师项目(2020GGJS249)
新乡市重大科技专项(2020238)。
