摘要
本试验旨在探究混合益生菌(凝结芽孢杆菌、植物乳杆菌、鼠李糖乳杆菌和嗜酸乳杆菌,活菌数≥1.75×10^(9) CFU/g)对大口黑鲈生长性能、肉品质及肠道健康的影响。选择990尾初始体重为(36.17±1.52)g大口黑鲈,随机分为3组,每组3个重复,每个重复110尾。各组分别饲喂基础饲料中添加0(对照组,CON组)、0.1%(MP-0.1%组)和1.0%(MP-1.0%组)的混合益生菌。试验期130 d。结果表明:1)各组之间生长性能和形体指标无显著差异(P>0.05)。2)与CON组相比,MP-0.1%和MP-1.0%组的肌肉纤维直径显著降低(P<0.05),MP-1.0%组的肌肉密度显著升高(P<0.05)。3)与CON组相比,MP-1.0%组的肌肉硬度、胶黏性和咀嚼性显著升高(P<0.05)。4)与CON组相比,MP-1.0%组的肌肉水分含量显著降低(P<0.05),肌肉粗蛋白质、风味氨基酸(鲜味氨基酸、甜味氨基酸)、多不饱和脂肪酸、omega-3脂肪酸及omega-6脂肪酸含量显著升高(P<0.05);MP-0.1%组的肌肉多不饱和脂肪酸和omega-3脂肪酸含量显著升高(P<0.05),而肌肉单不饱和脂肪酸含量显著下降(P<0.05)。5)与CON组相比,MP-0.1%和MP-1.0%组的肌肉中土臭素和2-甲基异莰醇含量均显著降低(P<0.05)。6)与CON组相比,MP-1.0%组的胃蛋白酶和胰蛋白酶活性均显著升高(P<0.05)。7)与CON组相比,MP-0.1%组的肠道绒毛宽度显著升高(P<0.05)。8)与CON组相比,MP-1.0%组的肠道异戊酸和总酸含量显著升高(P<0.05)。9)3组之间的肠道鲸杆菌属(Cetobacterium)和消化链球菌属(Peptostreptococcus)相对丰度具有显著差异(P<0.05),且MP-0.1%和MP-1.0%组的肠道菌群相似度更高,明显不同于CON组。由此可见,饲料中添加混合益生菌可改善大口黑鲈的肉品质及肠道健康。本试验条件下,大口黑鲈饲料中混合益生菌的适宜添加水平为1.0%。
The aim of this experiment was to investigate the effects of mixed probiotics(Bacillus coagulans,Lactobacillus plantarum,Lactobacillus rhamnosus and Lactobacillus acidophilus,and viable count≥1.75×10^(9) CFU/g)on the growth performance,meat quality and intestinal health of largemouth bass(Micropterus salmoides).A total of 990 largemouth bass with initial body weight of(36.17±1.52)g were randomly divided into 3 groups with 3 replicates in each group and 110 fish in each replicate.Fish in 3 groups were fed the basal diets supplemented with 0(control group,CON group),0.1%(MP-0.1%group)and 1.0%(MP-1.0%group)mixed probiotics,respectively.The experiment lasted for 130 days.The results showed as follows:1)there were no significant differences in growth performance and shape indexes among all groups(P>0.05).2)Compared with the CON group,the myofiber diameter of MP-0.1%and MP-1.0%groups was significantly decreased(P<0.05),and the myofiber density of MP-1.0%group was significantly increased(P<0.05).3)Compared with the CON group,the muscle hardness,gumminess and chewiness of MP-1.0%group were significantly increased(P<0.05).4)Compared with the CON group,the muscle moisture content of MP-1.0%group was significantly decreased(P<0.05),and the contents of crude protein,flavor amino acids(delicious amino acids and sweet amino acids),polyunsaturated fatty acids,omega-3 fatty acids and omega-6 fatty acids in muscle were significantly increased(P<0.05);the contents of polyunsaturated fatty acids and omega-3 fatty acids in muscle of MP-0.1%group were significantly increased(P<0.05),and the muscle monounsaturated fatty acids content was significantly decreased(P<0.05).5)Compared with the CON group,the contents of geosmin and 2-methyli-soborneol in muscle of MP-0.1%and MP-1.0%groups were significantly decreased(P<0.05).6)Compared with the CON group,the activities of pepsin and trypsin in intestine of MP-1.0%group were significantly increased(P<0.05).7)Compared with the CON group,the intestinal villus width of MP-0.1%group was significantly increased(P<0.05).8)Compared with the CON group,the contents of isovaleric acid and total acid in intestine were significantly increased(P<0.05).9)The relative abundances of Cetobacterium and Peptostreptococcus in intestine had significantly different among three groups(P<0.05),and the MP-0.1%and MP-1.0%groups had more similar intestinal flora,which were different from the CON group.In conclusion,dietary mixed probiotics can improve the meat quality and intestinal health of largemouth bass.Under the conditions of this experiment,the dietary mixed probiotics appropriate supplemental level is 1.0%.
作者
周秀珍
刘滔
张毅
王扬
赵敏洁
王旭堂
黄菊
冯凤琴
ZHOU Xiuzhen;LIU Tao;ZHANG Yi;WANG Yang;ZHAO Minjie;WANG Xutang;HUANG Ju;FENG Fengqin(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316022,China;Xianghu Laboratory,Hangzhou 311203,China;Zhejiang Aquatic Product Technology Promotion General Station,Hangzhou 311110,China;College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,China;Hangzhou Longyu Bio-Technology Co.,Ltd.,Hangzhou 310030,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2024年第7期4588-4609,共22页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
浙江省科技计划项目—多营养层级高效水产养殖技术(2022C02040)。
关键词
混合益生菌
生长
肉品质
肠道健康
大口黑鲈
mixed probiotics
growth
meat quality
intestinal health
Micropterus salmoides
