摘要
目的:建立川木香标准汤剂的质量控制方法。方法:采用Waters CORTECS T3超高效液相色谱柱,以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,流速为0.35 mL·min^(-1),柱温为40℃,检测波长在0~21 min为325 nm、21~37 min为225 nm,进样体积为1μL。采用超高效液相色谱法建立14批川木香标准汤剂的指纹图谱,确定指纹图谱共有峰,采用对照品对共有峰进行确证;通过相似度计算和化学计量学方法对不同产地川木香制备的标准汤剂进行分析,并对其中木香烃内酯、去氢木香内酯、绿原酸和4,5-二-O-咖啡酰奎宁酸进行定量分析。结果:川木香标准汤剂指纹图谱共标记14个共有峰,指认了木香烃内酯、去氢木香内酯、绿原酸和4,5-二-O-咖啡酰奎宁酸4个成分;相似度评价、聚类分析和主成分分析均将14批川木香标准汤剂分成2类,正交偏最小二乘法-判别分析从14个共有峰中筛选出7个差异性标志物;含量测定结果显示,来自四川阿坝的川木香制备的标准汤剂中木香烃内酯和去氢木香内酯的总质量分数最高、绿原酸和4,5-二-O-咖啡酰奎宁酸的总质量分数最低,而其余3个产地川木香差异不大。结论:该方法分析时间短、准确度高、专属性好,能够为川木香标准汤剂及其相关制剂质量标准的制定提供参考。
Objective:To establish a quality control method for standard Vladimiriae Radix decoction.Methods:The ultra-high performance liquid chromatography(UPLC)column Waters CORTECS T3 was used.Gradient elution was carried out using acetonitrile-0.1%phosphoric acid solution as the mobile phase.The flow rate of the mobile phase was 0.35 mL·min^(-1),and the column temperature was 40℃.The detection wavelength was 325 nm at 0-21 min and 225 nm at 21-37 min.The chromatographic injection volume was 1μL.14 batches of standard Vladimiriae Radix decoction fingerprints were established by UPLC to determine the common peaks in the fingerprints.The common peaks were confirmed using reference substances.Similarity calculation and chemometrics methods were used to analyze the standard decoction prepared by Vladimiriae Radix from different regions,and four effective components,namely costunolide,dehydrocarbactone,chlorogenic acid,and 4,5-di-O-caffeoylquinic acid were quantitatively analyzed.Results:A total of 14 common peaks were identified in the fingerprints of standard Vladimiriae Radix decoction,and four components were identified:costunolide,dehydrocarbactone,chlorogenic acid,and 4,5-di-O-caffeoylquinic acid.Similarity evaluation,cluster analysis(HCA),and principal component analysis(PCA)all divided the 14 batches of standard Vladimiriae Radix decoction into two categories,and orthogonal partial least squares-discriminant analysis(OPLS-DA)screened a total of seven differential markers from the 14 common peaks.The content determination results showed that the standard decoction prepared by Vladimiriae Radix from Aba,Sichuan,had the highest total content of costunolide and dehydrocarbactone,while the total content of chlorogenic acid and 4,5-di-O-caffeoyl quinic acid was the lowest.The overall difference between Vladimiriae Radix from the other three regions was not significant.Conclusion:This method has a short analysis time,high accuracy,and good specificity,and it can provide a reference for the formulation of quality standards for standard Vladimiriae Radix decoction and its related preparations.
作者
李振雨
梁月仪
卢晓莹
吕渭升
何民友
何广铭
刘晓霞
夏天睿
陈志鹏
黄爽
潘礼业
LI Zhen-yu;LIANG Yue-yi;LU Xiao-ying;LYU Wei-sheng;HE Min-you;HE Guang-ming;LIU Xiao-xia;XIA Tian-rui;CHEN Zhi-peng;HUANG Shuang;PAN Li-ye(Guangdong Yifang Pharmaceutical Co.,Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528244,China)
出处
《中国现代中药》
CAS
2024年第5期862-868,共7页
Modern Chinese Medicine
基金
中华人民共和国工业和信息化部2022年产业技术基础公共服务平台项目(2022-230-221)。
关键词
川木香
标准汤剂
超高效液相色谱法
指纹图谱
多指标成分定量
Vladimiriae Radix
standard decoction
UPLC
fingerprint
multiple indicator component quantification
