摘要
目的:建立川木香的质量标准。方法:采用硝酸银薄层和HPLC法对川木香进行了定性、定量分析。薄层色谱条件:2.5%硝酸银薄层板,展开剂环己烷-乙酸乙酯-丙酮(8∶1∶1),喷5%香草醛-浓硫酸溶液在105℃烘至显色。定量分析:色谱柱为C18柱,流动相为甲醇-水(70∶30),检测波长220nm。结果:采用硝酸银薄层鉴别的分离效果明显优于硅胶G薄层。含量测定中去氢木香内酯的平均回收率为97.51%;RSD值为2.38%。结论:所建立的定性鉴别和定量方法可用于控制川木香的质量。
Objective： To establish the Quality Standard of Radix Vladimiriae. Methods： The quality was determined by TLC and the content was determined by HPLC. The conditions of TLC were： the 2.5% silver nitrate folium, developer agent： cyclohexane - ethyl acetate - acetone （8 ： 1 ： 1 ） ; Developer： the solution of 5 % vaniUic aldehyde - concentrated sulfuric acid, displaying color in 105℃. The conditions of HPLC were： C18 eolunm, methanol - water （70 ：30） as the mobile phase, wavelength set at 220nm. Results： The TLC indicated that in the silver nitrate folium, the separate level of eostunolide and dehydrocostuslactone was higher than that in the silica gel folium. In the content determi- nation, the average recovery of dehydrostuslaetone was 97.51% （RSD = 2.38%）. Conclusion： This article established the methods of TLC and HPLC for Radix Vladimiriae, which could be used for controlling the quality of Radix Vladimiriae.
出处
《成都中医药大学学报》
2006年第1期54-56,共3页
Journal of Chengdu University of Traditional Chinese Medicine
基金
四川省中医管理局课题(编号:2003A06)
