摘要
重楼药材的供不应求促进了对其基原植物地上部分废弃资源的综合利用与开发研究。前期基于液质联用技术明确了华重楼地上部分的化学组成,并从中分离得到一系列黄酮和甾体皂苷类成分,该研究继续针对基于液质联用技术发现的潜在新化合物进行分离和结构鉴定研究。采用硅胶、ODS、Flash快速制备等柱色谱技术,结合制备高效液相色谱,从华重楼地上部分75%乙醇提取物分离得到5个化合物,通过波谱数据结合化学转化的方法鉴定了5个化合物的结构,分别为(23S,25R)-23α,27-二羟基薯蓣皂苷元-3-O-α-L-吡喃鼠李糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖苷(1)、(25R)-26-O-β-D-吡喃葡萄糖基-呋甾-5-烯-3β,22α,26-三醇-3-O-α-L-吡喃鼠李糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→4)-α-L-吡喃鼠李糖基-(1→4)]-β-D-吡喃葡萄糖苷(2)、(25R)-27-O-β-D-吡喃葡萄糖基-5-烯-3β,27-二羟基螺甾-3-O-α-L-吡喃鼠李糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→4)-α-L-吡喃鼠李糖基-(1→4)]-β-D-吡喃葡萄糖苷(3)、(25R)-27-O-β-D-吡喃葡萄糖基-5-烯-3β,27-二羟基螺甾-3-O-α-L-吡喃鼠李糖基-(1→2)-[(β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖苷(4)和aculeatiside A(5),其中化合物1~4为新化合物,化合物5为首次从本植物中分离得到。
The shortage of Paridis Rhizoma promotes comprehensive utilization and development research of waste aerial parts of the original plant.The chemical compositions of the aerial parts of Paris polyphylla var.chinensis were clarified based on the ultrahigh performance liquid chromatography tandem quadrupoles time of flight mass spectrometry(UPLC-QTOF-MS/MS)in the previous investigation,and a series of flavonoids and steroidal saponins were isolated.The present study continued the isolation and structure identification of the new potential compounds discovered based on UPLC-QTOF-MS/MS.By using silica gel,ODS,flash rapid preparation,and other column chromatography techniques,combined with prepared high performance liquid chromatography,five compounds were isolated from the 75%ethanol extract of the aerial parts of P.polyphylla var.chinensis,and their structures were identified by spectral data combined with chemical transformations,respectively,as(23S,25R)-23,27-dihydroxy-diosgenin-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranoside(1),(25R)-26-O-β-D-glucopyranosyl-furost-5-en-3β,22α,26-triol-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(2),(25R)-27-O-β-D-glucopyranosyl-5-en-3β,27-dihydroxyspirost-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(3),(25R)-27-O-β-D-glucopyranosyl-5-en-3β,27-dihydroxyspirost-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranoside(4),and aculeatiside A(5).Among them,compounds 1-4 were new ones,and compound 5 was isolated from P.polyphylla var.chinensis for the first time.
作者
郑子璐
谭晓敏
关亮俊
王茹
陈两绵
王智民
高慧敏
ZHENG Zi-lu;TAN Xiao-min;GUAN Liang-jun;WANG Ru;CHEN Liang-mian;WANG Zhi-min;GAO Hui-min(National Engineering Laboratory of Quality Control Technology of Chinese Materia Medica,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2023年第17期4589-4597,共9页
China Journal of Chinese Materia Medica
基金
中国中医科学院科技创新工程项目(CI2021A04401,CI2021A04408)
国家自然科学基金项目(82173996)
国家重点研发计划“中医药现代化”专项(2017YFC1701900)。
关键词
重楼
华重楼
非药用部位
甾体皂苷
Paridis Rhizoma
Paris polyphylla var.chinensis
non-medicinal parts
steroidal saponins
