摘要
【目的】研究基于microRNA(miRNA)高通量测序技术鉴定蛋鸭在笼养急性应激情况下垂体miRNA的表达特性,旨在探索miRNA在应激情况下的潜在功能,为进一步探究龙岩山麻鸭在笼养过程中产生急性应激的分子机制提供参考。【方法】试验选取20只110 d龙岩山麻鸭,随机分组为笼养急性应激试验组(C组),即叠层笼上笼3 d状态下龙岩山麻鸭,和非应激对照组(FW组),即半舍饲散养龙岩山麻鸭;收集113 d时C组和FW组各4只龙岩山麻鸭垂体组织样品(C_P,FW_P),进行miRNA测序和分析。【结果】C_P和FW_P两组文库共检测到331个已知的miRNAs,预测了5个新miRNAs。差异分析结果显示,C_P组相对于FW_P组有8个miRNAs显著差异表达(P<0.05),其中4个miRNAs上调表达,4个miRNAs下调表达。差异表达miRNA的靶基因富集分析结果显示,靶基因极显著富集到钙信号通路(Calcium signaling pathway)、GnRH信号通路(GnRH signaling pathway)、孕酮介导的卵母细胞成熟(Progesterone−mediated oocyte maturation)等信号通路上(P<0.01)。【结论】笼养急性应激引起龙岩山麻鸭垂体组织中hsa-miR-129-1-3p_1ss20TG、hsa-let-7c-5p、hsa-miR-27a-3p_R-2、hsa-miR-99a-5p_R-1、hsa-let-7b-5p、hsa-miR-15b-5p_R+1_2ss20AG21CT、PC-5p-1405_462、hsa-let-7f-1-3p_1ss22CT 8个miRNAs的差异表达,以上miRNAs可能通过调控多种信号通路调节龙岩山麻鸭的应激、生长、繁殖等生命活动。
[Objective]Based on the high-throughput sequencing technology of microRNA(miRNA),this study aims to identify the expression characteristics of pituitary miRNA in egg-laying ducks under acute stress in cage and explore the potential function of miRNA under stress,thus providing a reference for further exploring the molecular mechanism of acute stress in Longyanshan hemp ducks during cage rearing.[Method]In this study,twenty 110-day-old Longyan shan hemp ducks were chosen.The ducks were randomly assigned into two groups:cage-reared(C group,n=10),which was subjected to a stacked cage system for three consecutive days,and free-range non-stressed group(FW group,n=10),which was raised under semihouseing conditions.At 113 days of rearing,four pituitary tissue samples were collected from each group(C_P,FW_P)for miRNA sequencing and analysis.[Result]A total of 331 known miRNAs were detected in the C_P and FW_P libraries,and 5 new miRNAs were predicted.Differential analysis revealed eight significantly differentially expressed miRNAs in C_P vs FW_P(P<0.05),with four miRNAs upregulated and four miRNAs downregulated.Enrichment analysis of target genes indicated significant enrichment in calcium signaling pathway,gonadotropin-releasing hormone signaling pathway(GnRH signaling pathway),progesteronemediated oocyte maturation and other signaling pathways(P<0.01).[Conclusion]These results suggest that acute stress in cages leads to differential expression of hsa-miR-129-1-3p_1ss20TG,hsa-let-7c-5p,hsamiR-27a-3p_R-2,hsa-miR-99a-5p_R-1,hsa-let-7b-5p,hsa-miR-15b-5p_R+1_2ss20AG21CT,PC-5p-1405_462,hsa-let-7f-1-3p_1ss22CT miRNAs in pituitary tissue,which may regulate stress,growth,reproduction,and other life activities of Longyan shan hemp ducks through various signaling pathways.
作者
张文韬
刘水兵
蒋红霞
周明芳
熊婷
刘秋红
胡晓龙
毛辉荣
刘三凤
陈彪
ZHANG Wentao;LIU Shuibing;JIANG Hongxia;ZHOU Mingfang;XIONG Ting;LIU Qiuhong;HU Xiaolong;MAO Huirong;LIU Sanfeng;CHEN Biao(College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China;Poultry Research Institute,Jiangxi Agricultural University,Nanchang 330045,China;Jiangxi Biotech Vocational College,Nanchang 330200,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2023年第4期931-943,共13页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金项目(32102541)
江西省自然科学基金项目(20212BAB215016)
江西省现代农业家禽产业技术体系(JXARS-09)。
关键词
龙岩山麻鸭
笼养
应激
垂体
microRNA测序
Longyan shan hemp duck
cage rearing
stress reponse
pituitary
microRNA sequencing
