摘要
探究固态酿造浓香型白酒(NX)和酱香型白酒(JX)抑制肝癌细胞HepG2增殖的作用机制。采用细胞计数试剂盒8(CCK8)法评价细胞增殖,用试剂盒检测乙醇脱氢酶(ADH)、过氧化氢酶(CAT)、黄嘌呤氧化酶(XOD)的酶活以及活性氧(ROS)和一氧化氮(NO)的含量评价HepG2氧化应激酶的水平,采用实时定量PCR检测氧化应激关键基因核因子E2相关因子2(NRF2)及其下游基因醌氧化还原酶(NQO1)的表达。结果:100 mmol/L乙醇(ETOH)浓度时NX和JX均能显著抑制HepG2的增殖,抑制率达15%;JX(6.25~100 mmol/L ETOH)显著上调HepG2细胞内XOD和CAT的活力。ROS检测发现在12.5 mmol/L ETOH时其含量达到峰值,且受JX影响较大。NX(6.25~100 mmol/L ETOH)较JX显著促进NO的生成。NX和JX(100 mmol/L ETOH)均可显著抑制HepG2胞内NRF2及其下游基因HQO-1的表达。用抗氧化剂N-乙酰-L-半胱氨酸(NAC)抑制氧化应激反应后,NX和JX抑制HepG2增殖的作用均消失,同时也阻断其对NRF2和HQO-1表达的调控。结论:NX和JX均通过促进氧化应激抑制肝癌HepG2细胞的增殖。
The aim of this study was to explore the inhibitory mechanism of solid-state fermented Chinese alcoholic beverage Luzhou-flavor baijiu(NX)and Maotai-flavor baijiu(JX)on the cell proliferation of hepatoma cell line HepG2.CCK-8(Cell Counting Kit 8)method was used to evaluate the cell proliferation of HepG2.The activity of alcohol dehydrogenase(ADH),catalase(CAT),xanthine oxidase(XOD)and the contents of reactive oxygen species(ROS)and nitric oxide(NO)were detected by kit to evaluate the oxidative stress response.The gene expression level of Nuclear factor erythroid 2-related factor 2(NRF2)and NAD(P)H quinone oxidoreductase 1(NQO1)was measured by real-time quantitative PCR(q RT-PCR).Results:Compared with 100 mmol/L ethanol,NX and JX significantly inhibited HepG2 proliferation.JX(6.25-100 mmol/L)had a higher promoting effect than NX on regulation of the XOD and CAT.However,NX(6.25-100 mmol/L)significantly enhanced the production of NO than JX.Both NX and JX(100 mmol/L)decreased the gene expression of NRF2 and NQO1 in HepG2.Treatment with the antioxidant N-acetylcysteine(NAC)abolished the inhibition of cell proliferation and the down-regulation of NRF2 and NQO1 induced by both JX and NX.Conclusion:Both Luzhou-flavor and Maotai-flavor baijiu may inhibit the proliferation of hepatoma cell line HepG2 through oxidative stress.
作者
周琦
管祺杰
耿燕
Zhou Qi;Guan Qijie;Geng Yan(School of Life Sciences and Health Engineering,Jiangnan University,Wuxi 214122,Jiangsu;National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing,Jiangnan University,Wuxi 214122,Jiangsu)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2023年第8期60-66,共7页
Journal of Chinese Institute Of Food Science and Technology
关键词
中国白酒
肝癌
氧化应激
细胞模型
Chinese Baijiu
hepatocellular carcinoma
oxidative stress
cell model
