摘要
选取48只小白鼠,随机分为8组,每组6只,1组为对照组,2、3、4组为给药组,5组为照射组,6、7、8组为照射给药组,1~4组不照射,5~8组用5 Gy^(60)Coγ射线照射,照后30~60 min,1、5组和2~4、6~8组分别灌胃5 d蒸馏水和蕨麻多糖,第6天处死小鼠,采集血液、肝脏、脾脏和股骨进行检测。结果显示,蕨麻多糖可以使辐射小鼠红细胞和白细胞数量、血红蛋白含量升高、红细胞比积增大,使脾脏指数、总抗氧化能力(T-AOC)、超氧化物岐化酶(SOD)的活性、淋巴细胞亚群比例上升,促进丙二醛(MDA)含量降低,一定程度提升辐射小鼠的DNA系数。蕨麻多糖对辐射损伤小鼠的造血系统、抗氧化系统和骨髓细胞DNA损伤具有较好的治疗效果。
Forty-eight mice were randomly divided into eight groups of six mice each.Group 1 was the control group,groups 2,3,and 4 were the Potentilla anserina L drug groups,group 5 was the irradiation group,and groups 6,7,and 8 were the irradiated Potentilla anserina L drug groups.The control group was administered distilled water by gavage for 5 d without irradiation;the irradiation groups were irradiated with 5 Gy^(60)Coγrays.In the drug groups,the mice were gavaged with distilled water and Potentilla anserina L polysaccharide within 30-60 min after irradiation for 5 d and euthanized on the sixth day.The effects of different doses of Potentilla anserina L polysaccharide on the blood routine,organ index,DNA content in bone marrow,superoxide dismutase(SOD)activity,total antioxidant capacity(T-AOC),lymphocyte subpopulation ratio,and malondialdehyde(MDA)content in the mouse livers were determined.The results showed that Potentilla anserina L polysaccharide increased the erythrocyte and leukocyte counts,hemoglobin content,and erythrocyte-specific volume as well as the spleen index,liver T-AOC,SOD activity,and lymphocyte count and decreased the MDA content.The DNA coefficient of irradiated mice was improved to some extent.Potentilla anserina L polysaccharide exert good therapeutic effect on the hematopoietic and antioxidant systems and can mitigate the DNA damage of bone marrow cells in radiationinjured mice.
作者
吴代燕
冯洋洋
张琳梓
刘前虔
李杭雨
央尖珍玛
封海波
WU Daiyan;FENG Yangyang;ZHANG Linzi;LIU Qianqian;LI Hangyu;YANG Jianzhenma;FENG Haibo(College of Animal Husbandry and Veterinary Medicine,Southwest Minzu University,Chengdu 610041,China)
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2023年第1期74-82,共9页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金面上项目(31872511)
四川省应用基础研究计划项目(2021YJ0289)
引进人才科研启动金资助项目(RQD2021075)资助。
关键词
^(60)Coγ射线
蕨麻多糖
造血系统
抗氧化功能
^(60)Coγray
Potentilla anserina L polysaccharide
Hematopoietic system
Antioxidant system
