摘要
目的:建立一种高效液相色谱法同时测定葵花籽中苯甲酸、山梨酸、糖精钠3种食品添加剂的检测方法。方法:利用Agilent ZORBAX SB-C18色谱柱(250 mm*4.6 mm,5μm)、可变波长紫外检测器(VWD)进行检测,进样量:10μL;流速1.0 mL/min;检测波长:230 nm;甲醇—0.02 mol/L乙酸铵溶液为流动相;外标法进行定量分析。通过优化高效液相色谱条件,确定流动相,设置流动相的不同比例、不同流速,确定检测波长,进行分析。结果:苯甲酸、山梨酸、糖精钠3种添加剂可以得到很好的分离,在1.0~100.0μg/mL范围内具有良好的线性关系,相关系数均大于0.99,加标回收率为:96.14%~99.36%,相对标准偏差(RSD)为:0.02%~0.04%。结论:本实验方法简单高效,重复性好,操作容易,可以用于食品中葵花籽的山梨酸、苯甲酸、糖精钠3种食品添加剂的含量测定。
Objective :To establish a HPLC method for the simultaneous determination of benzoic acid,sorbic acid and saccharin sodium in sunflower seeds. Methods:Agilent ZORBAX SB-C18 column(250 mm*4.6 mm,5 μm)and variable wavelength ultraviolet detector(VWD)were used for detection,injection volume :10 μL;flow rate :1.0 mL/min;detection wavelength :230 nm;methanol-0.02 mol/L ammonium acetate solution as mobile phase;quantitative analysis was carried out by external standard method. By optimizing the conditions of HPLC,the mobile phase was determined,different proportion and flow rate of mobile phase was set,the detection wavelength was determined for analysis. Results :Benzoic acid,sorbic acid and saccharin sodium could be well separated,there was a good linear relationship in the concentration range of 1.0 ~ 100.0 μg/mL. The correlation coefficients were greater than 0.99,the recovery was 96.14% ~ 99.36%,and the relative standard deviation(RSD)was 0.02% ~ 0.04%.Conclusion :The experimental method is simple,efficient,reproducible and easy to operate. It can be used for the determination of sorbic acid,benzoic acid and saccharin sodium in sunflower seeds.
作者
崔婷婷
张淑芳
俎爱忠
CUI Tingting;ZHANG Shufang;ZU Aizhong(Ulanqab Inspection and Testing Center,Ulanqab 012000;Ulanqab Product Quality Measurement,Inspection and Testing Center,Ulanqab 012000)
出处
《中国食品添加剂》
CAS
北大核心
2023年第2期283-288,共6页
China Food Additives
关键词
高效液相色谱
山梨酸
苯甲酸
糖精钠
葵花籽
high performance liquid chromatography
sorbic acid
benzoic acid
saccharin sodium
sunflower seeds
