摘要
为探索针对设施番茄青枯病的高效绿色防治方法,从设施番茄菜地采集发病土壤,并添加生物改良剂腐殖酸和(或)壳聚糖,在26℃的恒温培养箱内进行为期10周的土壤培育试验。结果表明:腐殖酸与壳聚糖配施处理(HC)下,土壤细菌群落结构显著区别于空白对照和单施处理(P<0.05);HC处理下青枯病病原菌劳尔氏菌(Ralstonia spp.)的相对丰度显著降低,有益微生物链霉菌属(Streptomyces spp.)、北里孢菌属(Kitasatospora spp.)、细链孢菌属(Catenulispora spp.)和Terracidiphilus spp.的相对丰度显著增加(P<0.05);共现网络结果表明HC处理下子网络的网络连接性、聚类系数和平均度显著提高(P<0.05),有益微生物与劳尔氏菌间存在直接和间接的抑制作用,且以间接抑制作用为主。研究表明,腐殖酸与壳聚糖配施增强了土壤细菌群落之间的相互作用,并且能通过富集有益微生物类群来抑制劳尔氏菌生长,在合理管理设施番茄菜地、防控土传病害中具有实际应用潜力。
In order to explore more efficient green control methods for bacterial wilt in tomato facilities,we collected infected soil,added biological modifiers,humic acid and/or chitosan,and incubated it at 26℃for 10 weeks.The results showed that the soil bacterial community structure was significantly different with the co-application of humic acid and chitosan(HC)compared with the control and single application treatments(P<0.05).Under HC treatment,the relative abundance of the bacterial wilt pathogen Ralstonia spp.was significantly decreased,while the relative abundance of beneficial microorganisms,Streptomyces spp.,Kitasatospora spp.,Catenulispora spp.,and Terracidiphilus spp.,was significantly increased(P<0.05).The co-occurrence network results showed that the network connectivity,clustering coefficient,and average degree of HC subnetworks were significantly improved(P<0.05)and there were direct and indirect inhibitory effects between beneficial microorganisms and Ralstonia spp.;the indirect inhibitory effect was dominant.Our results indicated that the co-application of humic acid and chitosan enhanced the interaction between soil bacterial communities and inhibited the growth of Ralstonia spp.by promoting beneficial microbial groups.This has practical application potential in the reasonable management of tomato facility fields and the control of soil-borne diseases.
作者
鲍莹
严梦圆
吴萌
李桂龙
刘凯
李忠佩
BAO Ying;YAN Mengyuan;WU Meng;LI Guilong;LIU Kai;LI Zhongpei(State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2022年第12期2772-2778,共7页
Journal of Agro-Environment Science
基金
国家自然科学基金面上项目(32071642)
国家重点研发计划项目(2021YFC1808901)。
关键词
腐殖酸
壳聚糖
设施番茄
青枯病
高通量测序
humic acid
chitosan
tomato facility
bacterial wilt
high-throughput sequencing
