摘要
为探索西瓜连作土壤改良方法,采用放线菌秸秆降解液处理西瓜连作土壤,设置清水(CK)、秸秆(J)、放线菌(F)、秸秆+放线菌(JF)等4个处理,测定分析不同处理对土壤化学性质、酶活性、微生物含量及西瓜幼苗生长发育的影响,并对不同处理土壤进行高通量测序,研究其微生物群落结构变化。结果表明,与CK相比,JF处理土壤的p H、有机质含量、氧化还原酶(脲酶、转化酶、过氧化氢酶)活性和细菌数量显著提高,真菌数量显著降低。与CK相比,J、F、JF处理显著提升了土壤微生物群落丰富度,4个处理的真菌、细菌群落区分度较为明显。与CK、J、F处理相比,JF处理显著提高了西瓜幼苗的壮苗指数。推测枝孢菌、毛壳菌及芽孢杆菌、放线菌等共同作用改良了西瓜连作土壤生态结构。研究结果为西瓜连作土壤改良及西瓜产业健康可持续发展提供了理论依据和实践支持。
To explore the soil improvement methods for continuous watermelon cropping,the soil for continuous watermelon cropping was treated with actinomycetes straw degradation solution.Four treatments,namely CK,straw(J),actinomycetes(F),and straw+actinomycetes(JF),were set up.The effects of different treatments on soil chemical properties,enzyme activities,microbial content,and the growth and development of watermelon seedlings were determined and analyzed.High-throughput sequencing was conducted on different treated soils to study the changes in their microbial community structure.The results showed that compared with CK,the pH,organic matter content,REDOX enzyme(urease,invertase,catalase)activity and bacterial count of the soil treated with JF were significantly increased,while the fungal count was significantly decreased.Compared with CK,treatments J,F and JF significantly enhanced the richness of soil microbial communities,and the differentiation of fungal and bacterial communities in the four treatments was relatively obvious.Compared with the CK,J and F treatments,the JF treatment significantly increased the strong seedling index of watermelon seedlings.It is speculated that Cladosporium,Trichoderma,Bacillus and actinomycetes work together to improve the soil ecological structure of watermelon continuous cropping.The research results provide theoretical basis and practical support for soil improvement in continuous watermelon cropping and the healthy and sustainable development of the watermelon industry.
作者
贾云鹤
王喜庆
闫闻
付永凯
尤海波
王丽冬
刘晓飞
刘超
JIA Yunhe;WANG Xiqing;YAN Wen;FU Yongkai;YOU Haibo;WANG Lidong;LIU Xiaofei;LIU Chao(Horticulture Branch,Heilongjiang Academy of Agricultural Sciences,Harbin 150069,Heilongjiang,China;Harbin Commercial University,Harbin 150028,Heilongjiang,China)
出处
《中国瓜菜》
北大核心
2025年第8期101-110,共10页
China Cucurbits And Vegetables
基金
黑龙江省重点研发计划项目(SC2022ZX02C0202)
黑龙江省省属科研业务费项目(CZKYF2025-1-B016)
国家西甜瓜产业技术体系哈尔滨综合试验站(CARS-25)
黑龙江省西甜瓜现代农业产业技术协同创新推广体系项目。
关键词
西瓜
放线菌
秸秆
连作
土壤
Watermelon
Actinomycetes
Straw
Continuous cropping
Soil
