摘要
为了研究太子参参须皂苷(Pseudostellaria heterophylla fibrous root saponins,PHFRS)对免疫抑制小鼠免疫调节作用的影响,试验将80只雄性ICR小鼠随机分为5组,即空白对照(CK)组、环磷酰胺(CTX)组和PHFRS低剂量(L-PHFRS,50 mg/kg)、中剂量(M-PHFRS,100 mg/kg)、高剂量(H-PHFRS,200 mg/kg)组。第1~3天,采用腹腔注射法对CK组注射生理盐水,其余组均腹腔注射CTX(按体重80 mg/kg)。第4~17天,PHFRS各剂量组分别灌服相应剂量PHFRS,CK组、CTX组灌服等量纯化水。第18天采样,采用MTT法检测小鼠脾T、B淋巴细胞增殖情况,计算刺激指数(SI),采用免疫比浊法检测血清免疫球蛋白(IgA、IgM、IgG)、补体(C3、C4)含量,采用ELISA法检测血清细胞因子(IL-2、IL-4、IL-6、IFN-γ、TNF-α)含量。结果表明:与CK组相比,CTX组T淋巴细胞SI、IgM含量均显著降低(P<0.05),B淋巴细胞SI及IgA、C3、C4、IL-4含量均显著升高(P<0.05);M-PHFRS组C3含量显著升高(P<0.05);H-PHFRS组C3、C4、TNF-α含量显著升高(P<0.05)。在三种PHFRS浓度梯度内,T淋巴细胞SI呈剂量依赖性升高,B淋巴细胞SI无明显剂量依赖性,血清免疫球蛋白IgA、IgM、IgG和补体C3、C4含量均呈剂量依赖性升高,血清细胞因子除IL-6含量呈剂量依赖性升高外,IL-2、IL-4、TNF-α和IFN-γ含量呈现不同程度的升高或降低。说明PHFRS能够促进免疫抑制小鼠脾脏淋巴细胞增殖功能的恢复,调节血清中免疫球蛋白、补体及细胞因子水平,从而促进小鼠免疫功能的恢复,此外,PHFRS剂量的变化对免疫调节效果具有一定程度的影响。
In order to investigate the effects of Pseudostellaria heterophylla fibrous root saponins(PHFRS)on immunomodulatory function in immunosuppressed mice,in the experiment,80 male ICR mice were randomly divided into 5 groups,namely,blank control(CK)group,cyclophosphamide(CTX)group,and PHFRS low(L-PHFRS,50 mg/kg),medium(M-PHFRS,100 mg/kg),and high(H-PHFRS,200 mg/kg)groups.On days 1 to 3,normal saline was injected into the CK group by intraperitoneal injection,and CTX(80 mg/kg by body weight)was injected intraperitoneally into all the remaining groups.On days 4 to 17,each PHFRS dosage group was fed with the corresponding dose of PHFRS,and the CK and CTX groups were given equal amount of purified water.On the 18 th day of sampling,the proliferation of spleen T and B lymphocytes was detected by MTT method and the stimulation index(SI)was calculated;serum immunoglobulins(IgA,IgM,IgG)and complements(C3,C4)were detected by immunoturbidimetric method;serum cytokine(IL-2,IL-4,IL-6,IFN-γ,TNF-α)were detected by ELISA method.The results showed that compared with the CK group,the T-lymphocyte SI and IgM content were significantly lower(P<0.05)and the B-lymphocyte SI,IgA,C3,C4 and IL-4 contents were significantly higher(P<0.05)in the CTX group;C3 content was significantly higher in the M-PHFRS group(P<0.05);C3,C4,and TNF-αcontents were significantly increased in the H-PHFRS group(P<0.05).Within the three PHFRS concentration gradients,T-lymphocyte SI was dose-dependently increased,while B-lymphocyte SI showed no significant dose-dependence;serum immunoglobulins IgA,IgM,IgG and complements C3 and C4 levels were dose-dependently increased;among serum cytokines,except IL-6 showing dose-dependently increased level,IL-2,IL-4,TNF-αand IFN-γlevels showed different degrees of increase or decrease.The results indicated that PHFRS could promote the recovery of spleen lymphocyte proliferation function in immunosuppressed mice,and regulate the levels of immunoglobulins,complements and cytokines in serum,thus promoting the recovery of immune function in mice;in addition,the change of PHFRS dose had a certain degree of influence on the immunomodulatory effect.
作者
于丛爽
陈俊宇
周梦圆
曾靖棋
黄钲
张炎达
马玉芳
YU Congshuang;CHEN Junyu;ZHOU Mengyuan;ZENG Jingqi;HUANG Zheng;ZHANG Yanda;MA Yufang(College of Animal Sciences(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Provincial Key Laboratory of Chinese-Western Veterinary Medicine and Animal Health,Fuzhou 350002,China;Fujian Beidi Pharmaceutical Co.,Ltd.,Ningde 355399,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2022年第24期108-112,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
福建省重大专项专题项目(2021NZ02908)
福建农林大学大学生创新项目(202010389174)。
关键词
太子参
参须
皂苷
免疫抑制
小鼠
免疫调节
Pseudostellaria heterophylla
fibrous root
saponin
immunosuppression
mice
immunomodulation
