摘要
【目的】探明浙江凤阳山地带性植被常绿阔叶林土壤微生物群落特征,明确土壤微生物群落沿海拔梯度的变化规律及影响微生物群落结构和多样性的主要因子。【方法】采集海拔343、765、1 364、1 611 m处土壤样品,利用高通量测序技术,探究土壤微生物群落与海拔的关系。【结果】凤阳山细菌操作分类单元(OTU)数量多于真菌,中低海拔(343和765 m)共有的OTU数目较多;Chao 1指数随海拔上升呈下降趋势,Shannon指数没有明显的变化规律。在门水平上,细菌群落优势类群为酸杆菌门Acidobacteria (43.77%~51.55%)、变形菌门Proteobacteria (31.18%~35.77%)和放线菌门Actinobacteria (5.24%~7.99%);真菌群落优势类群为担子菌门Basidiomycota (33.16%~67.35%)和子囊菌门Ascomycota(22.98%~46.78%)。相对丰度前10位的细菌门中,芽单胞菌门Gemmatimonadetes、硝化螺旋菌门Nitrospirae、疣微菌门Verrucomicrobia与海拔呈极显著负相关(P<0.01)。真菌群落不存在与海拔相关的微生物门,而LefSe线性判别分析结果显示:真菌群落具有更多的差异类群。此外,主坐标分析显示:土壤微生物群落以765 m为界,存在海拔分异性特征,且第1主轴与温度、土壤全磷、土壤全钾、土壤pH显著相关(P<0.05)。【结论】海拔变化引起了凤阳山土壤微生物群落特征的变化,并且温度是最主要的驱动因子。图5表6参41。
[Objective] The objective is to explore the characteristics of soil microbial community of zonal vegetation in evergreen broad-leaved forest in Fengyang Mountain, Zhejiang Province, and to clarify the changes of soil microbial community along the elevation gradient and the main factors affecting structure and diversity of microbial community. [Method] Soil samples were collected at 343, 765, 1 364 and 1 611 m above sea level. High-throughput sequencing technology was used to explore the relationship between soil microbial community and altitude. [Result] The number of OTU of bacteria was more than that of fungi, and middle and low altitudes(343 and 765 m) displayed more OTUs. Chao 1 index decreased with the increase of altitude,while Shannon index had no obvious trend. The dominant taxa of bacteria at the phylum level were Acidobacteria(43.77%-51.55%), Proteobacteria(31.18%-35.77%) and Actinobacteria,(5.24%-7.99%), while the dominant groups of fungal community were Basidiomycota(33.16%-67.35%) and Ascomycota(22.98%-46.78%). Among the top 10 bacterial phyla in relative abundance, Gemmatimonadetes, Nitrospirae and Verrucomicrobia were significantly negatively correlated with altitude(P<0.01). There were no altitudinal taxa in the fungal community at the phylum level. LefSe(LDA Effect Size) analysis exhibited more different taxa in the fungal community. In addition, PCoA showed that the soil microbial community had the characteristics of altitudinal differentiation bounded by 765 m, and the first axis of this PcoA(PC1) was significantly correlated with temperature, total phosphorus, total kalium and pH(P<0.05). [Conclusion] The change of altitude leads to the change of soil microbial community characteristics in Fengyang Mountain, and temperature is the main driving factor. [Ch, 5 fig. 6 tab. 41 ref.]
作者
何柳
曹敏敏
鲁建兵
郑翔
刘胜龙
姜姜
HE Liu;CAO Minmin;LU Jianbing;ZHENG Xiang;LIU Shenglong;JIANG Jiang(Key Laboratory of Soil and Water Conservation and Ecology Restoration,Co-Innovation Center of Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Administration of Fengyang Mountain,Fengyang Mountain-Baishanzu National Nature Reserve,Longquan 323700,Zhejiang,China)
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2022年第6期1267-1277,共11页
Journal of Zhejiang A&F University
基金
国家自然科学基金资助项目(32071612)
国家重点研发计划项目(2017YFC0505500,2017YFC0505502)。
关键词
土壤细菌
土壤真菌
海拔
高通量测序
凤阳山
soil bacteria
soil fungi
altitude
high throughput sequencing
Fengyang Mountain
