摘要
为了解海拔对油松Pinus tabulaeformis根围土壤真菌多样性及群落结构的影响及探究真菌群落结构对土壤理化性质变化的响应,采用Illumina Miseq高通量测序平台对内蒙古乌拉山国家森林公园油松根围土壤真菌群落组成进行了研究。结果表明,4个海拔的土壤样本测序得到油松根围真菌有效序列共256991条,划分为1026个OTU,分属于13门、37纲、84目、145科、192属。在门水平上,油松根围优势真菌主要分布于子囊菌门Ascomycota、担子菌门Basidiomycota和被孢霉门Mortierellomycota;在属水平上,主要归属于青霉属Penicillium(14.4%)、被孢霉属Mortierella(7.6%)、双担菌属Geminibasidium(5.3%)、丝盖伞属Inocybe(1.8%)、枝孢霉属Cladosporium(1.6%)、棉革菌属Tomentella(1.6%)、异多棒菌属Xenopolyscytalum(1.4%)、镰刀菌属Fusarium(1.3%)、蜡蚧菌属Lecanicillium(1.3%)、土赤壳属Ilyonectria(1.1%)、热子囊菌属Thermoascus(1.0%)、蜡壳耳属Sebacina(1.0%)和一些未定名属。对油松根围土壤真菌的α多样性指数分析以及NMDS聚类结果的分析表明,海拔1700m和1800m的油松根围土壤真菌群落结构相似,而与1500m的真菌群落结构差异显著,在海拔相对较高的1700m–1800m的真菌多样性及丰度显著高于海拔较低的1500m处。对油松根围优势真菌与土壤理化性质的冗余(RDA)分析及相关性分析表明,土壤真菌的群落结构受土壤理化性质(硝态氮NN、含水量WC、速效钾AK、铵态氮AN、pH值、有效磷AP、含碳量CC和总氮TN)的显著影响,其中NN和WC对油松根围土壤真菌群落结构的影响最为显著。
The fungal diversity and community structure in rhizosphere soil of Pinus tabulaeformis in Wula Mountain were investigated,and the fungal community structure associated with the soil physical and chemical properties was explored.The fungal community composition was studied using high-throughput sequencing technology.A total of 256991 sequences of rhizosphere soil fungi was obtained,dividing into 1026 OTUs belonging to 13 phyla,37 classes,84 orders,145 families and 192 genera.At phylum level,Ascomycota,Basidiomycota and Mortierellomycota were dominant.At generic level,Penicillium(14.4%),Mortierella(7.6%),Geminibasidium(5.3%),Inocybe(1.8%),Cladosporium(1.6%),Tomentella(1.6%),Xenopolyscytalum(1.4%),Fusarium(1.3%),Lecanicillium(1.3%),Ilyonectria(1.1%),Thermoascus(1.0%),Sebacina(1.0%)and some unclassified genera were dominant.Analyses of alpha diversity index and NMDS showed that the fungal community structure at altitude of 1800m was similar to that at altitude of 1700m,but significantly different from that at altitude of 1500m.Species diversity and abundance at altitude of 1700–1800m were significantly higher than those at altitude of 1500m.RDA and correlation analysis of dominant fungi and soil physical and chemical properties showed that the community structure of soil fungi was significantly affected by soil physical and chemical properties,and nitrate nitrogen and water content were the main influencing factors.
作者
李敏
闫伟
LI Min;YAN Wei(College of Life Science and Technology,Inner Mongolia Normal University,Hohhot,Inner Mongolia 010022,China;College of Forestry,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2019年第11期1992-2006,共15页
Mycosystema
基金
国家自然科学基金(31760169)
内蒙古自治区自然科学基金面上项目(2017MS0310)
内蒙古自治区高等学校科学研究项目(NJZY16043)~~
关键词
高通量测序
可操作分类单元
真菌多样性
理化性质
主成分相关性分析
high-throughput sequencing
operational taxonomic unit
fungal diversity
physical and chemical properties
RDA correlation analysis
