摘要
目的:比较不同干燥方式对太子参挥发性成分的影响。方法:采用常温晾干、晒干、热风干燥(40、60、80℃)和真空冷冻干燥的方式处理太子参,通过气相色谱-离子迁移谱法(GC-IMS)分析挥发性成分的变化,样品在500 r·min^(-1)转速下80℃孵化15 min,进样针温度85℃,进样量200μL,载气程序为20 min内从2 mL线性上升到150 mL,IMS迁移管温度60℃,SE-54毛细管色谱柱(0.32 mm×30 m,0.25μm),柱温60℃,时间35 min。构建挥发性成分的差异谱图并进行主成分分析(PCA)。结果:在干燥后的太子参中鉴别出了37种挥发性成分,化合物的种类数量从多到少依次为酮类、醛类、醇类。不同干燥方式的太子参中挥发性成分存在一定差异,晒干、晾干和40℃热风干燥后挥发性成分相近,真空冷冻干燥与80℃热风干燥处理对太子参挥发性成分有较大的影响,且真空冷冻干燥样品中化合物种类最少。结论:建立了一种太子参挥发性成分的GC-IMS检测分析方法,具有高效无损、样品处理简单的特点,可实现不同干燥方式太子参样品的区分。40℃热风干燥可以有效保留太子参样品中的挥发性成分,实现与晒干和晾干样品相似的风味。
Objective:To compare the effects of different drying methods on volatile components of Pseudostellariae Radix.Method:The samples were dried by different methods,including air drying,sun drying,hot air drying(40,60,80℃)and vacuum freeze drying.Gas chromatography-ion mobility spectrometry(GC-IMS)was used to compare the changes of volatile components in the samples after different treatments.The samples were incubated at 80℃and 500 r·min^(-1) for 15 min,the injection temperature was85℃,the injection volume was 200μL,the flow rate of carrier gas was from 2 mL to 150 mL during 20 min,and the temperature of IMS detector was 60℃.SE-54 capillary column(0.32 mm×30 m,0.25μm)was used,the column temperature was 60℃,and the analysis time was 35 min.The differential spectra of volatile components were constructed and analyzed by principal component analysis(PCA).Result:A total of 37volatile components were identified from dried Pseudostellariae Radix.The number of compounds in descending order was ketones,aldehydes and alcohols.There were some differences in the volatile components in samples dried by different methods.And the volatile components in samples with sun drying,air drying and hot air drying at 40℃were similar,compared with other drying methods,vacuum freeze drying and hot air drying at 80℃had great effects on the volatile components of Pseudostellariae Radix,and the compounds in the samples with vacuum freeze drying were the least.Conclusion:In this study,GC-IMS for the detection and analysis of volatile components in Pseudostellariae Radix is established,which has the characteristics of high efficiency,nondestructive inspection and simple sample processing.This method can be used for the distinction of Pseudostellariae Radix dried by different methods.And hot air drying at 40℃can effectively retain the volatile components of Pseudostellariae Radix,and achieve similar flavor to samples with sun drying and air drying.
作者
王雨晨
张敏敏
马文雅
赵恒强
刘军
崔莉
王晓
WANG Yuchen;ZHANG Minmin;MA Wenya;ZHAO Hengqiang;LIU Jun;CUI Li;WANG Xiao(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Qilu University of Technology(Shandong Academy of Sciences),Shandong Analysis and Test Center,Jinan250014,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2022年第20期100-107,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中央本级重大增减支项目(2060302)
齐鲁工业大学(山东省科学院)科教产融合创新试点工程国际合作项目(2020KJCGH08)。
