摘要
Lonicerae japonicae Flos(LF)is a kind of healthcare food with hepatoprotective function.This study was designed to explore the spectrum-effect relationships between UPLC fingerprints and the hepatoprotective effects of LF.Fingerprints of ten batches of LF were established by UPLC-PDA.The inhibitory levels of AST and ALT were used as pharmacological indexes,and secoxyloganin,isochlorogenic acid A and isochlorogenic acid C were screened as hepatoprotective active compounds by grey relational analysis(GRA)and partial least squares regression analysis(PLSR).Caspase-3 was obtained by network pharmacology as a key target of hepatoprotective active compounds.Molecular docking is used to explore the interaction between small molecules and proteins.This work provided a general model of the combination of UPLC-PDA and hepatoprotective effect to study the spectrum-effect relationship of LF,which can be used to considerable methods and insight for the fundamental research of the material basis of similar healthcare food.
基金
financially supported by the National Natural Science Foundation of China (81973604, 81803690 and 81703684)
Special Funds from the Central Finance to Support the Development of Local Universities
the National Natural Science Foundation Matching Project (2018PT02)
the Innovative Talents Funding of Heilongjiang University of Chinese Medicine (2018RCD25)
the Postdoctoral Initial Fund of Heilongjiang Province (UNPYSCT 2017219)
the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (UNPYSCT-2017215)
the National Natural Science Foundation Matching Project (2017PT01)
the Natural Science Foundation of Heilongjiang Province (H2015037)
the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (2014bs05)
the Application Technology Research and Development Projects of Harbin Technology Bureau (2014RFQXJ149)
the Heilongjiang Postdoctoral Scientific Research Developmental Fund (LBH-Q16210 and LBH-Q17161)
the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (2013bs04)
the scientific research project of Heilongjiang Provincial Health Commission (20211313050171)
Heilongjiang Touyan Innovation Team Program
National Famous Old Traditional Chinese Medecine Experts Inheritance Studio Construction Program of National Administration of TCM ([2022]No.75)
