摘要
目的:研究冷暴露介导小鼠回肠机械屏障损伤及其机制。方法:将20只小鼠随机分为对照组和冷暴露组,每组10只。对照组和冷暴露组均置于(24±2)℃,湿度为40%的气候室内,冷暴露组小鼠每日移至(4±2)℃的气候室内3 h,连续冷暴露三周。三周后采集小鼠回肠组织,通过苏木素伊红染色、Masson染色观察小鼠回肠组织结构变化,通过Western blot检测回肠组织紧密连接、炎症细胞因子、核因子-κB通路的相关蛋白表达水平。结果:与对照组相比,冷暴露小鼠回肠组织环形肌层变薄,大量的炎症细胞浸润、绒毛长度变短,隐窝深度增大,出现组织纤维化;与对照组比较,冷暴露小鼠的回肠紧密连接相关蛋白表达水平均显著下调(P<0.05),白细胞介素-1β、白细胞介素-6、p-p65蛋白表达水平均显著上调(P<0.05)。结论:冷暴露能够损伤小鼠回肠组织紧密连接,破坏机械屏障的完整性,其机制与激活NF-κB信号通路,促进炎症反应的发生有关。
Objective:To study the mechanisms of cold exposure mediated ileum mechanical barrier injury in mice.Methods:Twenty mice were randomly divided into the control and cold exposure groups.Both the control and cold exposure groups were placed in the climate room with(24±2)℃and 40%humidity.The mice in the cold exposure group were moved to the climate room at(4±2)℃every day for 3 hours for three consecutive weeks.Three weeks later,the ileum tissues of mice were collected.Changes in ileum tissue structure were observed by hematoxylin-eosin staining and Masson staining.The related protein expression levels of the tight junction,inflammatory cytokines,and the NF-κB pathway were detected by Western blot.Results:Compared with the control group,the circular muscle layer of the ileum in cold exposed mice became thin,a large number of inflammatory cells infiltrated,the length of villi became short,the depth of recess was increased,and tissue fibrosis appeared.The expression levels of ideal tight junction-associated proteins in cold exposed mice were decreased significantly(P<0.05),while the protein expression levels of IL-1β,IL-6 and phosphorescent p65 were increased significantly(P<0.05).Conclusion:Cold exposure can damage the tight junction of the mouse ileum,destroy the integrity of the mechanical barrier and activate the NF-κB signaling pathway to promote the occurrence of the inflammatory response.
作者
刘萌萌
张
张紫薇
袁建彬
郭景茹
LIU Meng-meng;ZHANG Yan;ZHANG Zi-wei;YUAN Jian-bin;GUO Jing-ru(Heilongjiang Bayi Agricultural University,Daqing 163319,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2022年第3期279-283,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助项目(32172817)
黑龙江省自然科学基金资助项目(LH2020C081)
黑龙江八一农垦大学三横三纵资助项目(ZRCPY202026)
校科研启动基金(XDB201805)。
