摘要
目的:探讨五味子甲素对脂多糖诱导小鼠RAW264.7细胞的抗炎作用。方法:检测五味子甲素对脂多糖诱导小鼠RAW264.7细胞的增殖、凋亡、吞噬及细胞内白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、前列腺素E2(PGE2)和环氧合酶-2(COX-2)水平的影响。脂多糖诱导后加入不同浓度五味子甲素,CCK8法测定RAW264.7细胞增殖能力,中性红试剂检测诱导后RAW264.7细胞的吞噬能力。酶联免疫吸附试验(ELISA)法测定细胞中IL-1β、TNF-α、PGE2和COX-2水平。Annexin V-FITC/PI双染检测五味子甲素对诱导后RAW264.7细胞凋亡的影响。结果:五味子甲素可明显抑制脂多糖诱导小鼠RAW264.7细胞的增殖、吞噬能力及诱导刺激的细胞凋亡;ELISA法测定结果显示,五味子甲素可抑制RAW264.7细胞IL-1β、TNF-α、PGE2和COX-2的分泌。结论:五味子甲素对脂多糖诱导的小鼠RAW264.7细胞炎症具有明显的抑制作用,其机制可能与抑制炎症细胞的增殖、吞噬,减少炎症因子释放,减轻脂多糖诱导的细胞凋亡有关。
Objective:To probe into the anti-inflammatory effects of schisandrin A on lipopolysaccharide-induced RAW264.7 cells in mice.Methods:Effects of schisandrin A on proliferation,apoptosis and phagocytosis of lipopolysaccharide-induced RAW264.7 cells in mice and levels of interleukin(IL)-1β,tumor necrosis factor-α(TNF)-α,prostaglandin E2(PGE2)and cyclooxygenase(COX)-2 levels were detected.The proliferation of RAW264.7 cells was measured by CCK8 method and the phagocytosis of RAW264.7 cells was detected by neutral red reagent.IL-1β,TNF-α,PGE2 and COX-2 were determined by enzyme-linked immunosorbent assay(ELISA).Annexin V-FITC/PI double staining was used to detect the effects of schisandrin A on apoptosis of RAW264.7 cells after induction.Results:Schisandrin A could significantly inhibit the proliferation,phagocytosis and apoptosis of lipopolysaccharide-induced RAW264.7 cells in mice.ELISA results showed that schisandrin A could inhibit the secretion of IL-lβ,TNF-α,PGE2 and COX-2 in RAW264.7 cells.Conclusion:Schisandrin A has significant anti-inflammatory effects on lipopolysaccharide-induced RAW264.7 cells in mice,and its mechanism may be related to inhibiting the proliferation and phagocytosis of inflammatory cells,reducing the release of inflammatory factors,and reliefing the lipopolysaccharide-induced apoptosis.
作者
许红
陶小军
李若男
李鑫磊
徐志立
Xu Hong;Tao Xiaojun;Li Ruonan;Li Xinlei;Xu Zhili(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Liaoning Dalian 116600,China)
出处
《儿科药学杂志》
CAS
2022年第8期1-5,共5页
Journal of Pediatric Pharmacy
基金
辽宁省自然科学基金,编号2019-ZD-0441。
