摘要
目的 建立测定九味肝泰胶囊中大黄素含量的方法。方法 采用高效液相色谱法,色谱柱为Shim-pack VP-ODS C18(4.6 mm×150 mm,5μm),流动相为甲醇一水(76∶24),流速为1.0mL·min-1,检测波长为254 nm,进样量为20μL。结果 大黄素浓度在0.00125～0.04 mg·mL-1内峰面积与浓度呈良好的线性关系,r=0.99999,平均回收率为95.6％,大黄素与其相邻杂质峰的分离度符合要求。结论 本文建立的分析方法简便、重现性好,可用于九味肝泰胶囊中大黄素含量测定。
OBJECTIVE To develop a method to determine emodin in Jiuwei Gantai capsules. METHODS We used a RP-HPLC method with Shin-pack Vp-ODS column (5μm- 4. 6 mm×150 mm), and the mobile phase was the mixture of methanol-water (76 : 24), and the flow rate was 1. 0 mL·min-1 . with the detection wavelength of 254 nm. Injection volume: 20 μL. RESULTS The linear correlation was observed from 0. 00125 mg·mL-1 to 0. 040 mg ·mL-1 (r=0. 99999) . The average recovery was 95. 6% with good precision. Resolution between emodin and other peaks met the requirements. CONCLUSIONS The method is convenient, selective and reproducible for the determination of emodion in Jiuwei Gantai capsules.
出处
《中南药学》
CAS
2003年第4期234-236,共3页
Central South Pharmacy
