摘要
目的:建立蒙药沃森萨乌日乐的质量标准。方法:采用显微鉴别方法鉴别处方中的诃子、山柰;采用薄层色谱法(TLC)对沃森萨乌日乐中石菖蒲进行薄层色谱定性鉴别;采用高效液相色谱法(HPLC)测定沃森萨乌日乐中没食子酸的含量。结果:显微鉴别中各药材组织特征明显;TLC法中样品与对照药材色谱相对应位置上显相同颜色的斑点,阴性中无干扰。没食子酸对照品在23.5~705.9 ng范围内呈良好的线性关系。回归方程为Y=64795X+0.105,R^(2)=0.9999,平均回收率为100.85%。结论:确定了处方中石细胞(诃子)、淀粉粒(山柰)的显微鉴别;石菖蒲薄层色谱定性鉴别专属性强;没食子酸的含量测定方法专属性强,重现性好,灵敏度高,简便快捷,方中其他组分对其测定无干扰,故成功地建立了蒙药沃森萨乌日乐的质量标准。
Objective:To establish the quality standard of Mongolian medicine Wosensa Wurile.Methods:The microscopical identification method was used to identify myrobalan and Kaempferol.Thin layer chromatography(TLC)was used to identify Acorus tatarinowii.A method for determination of gallic acid in Watson Saurilol by high performance liquid chromatography(HPLC)was established.Results:The tissue characteristics of each medicinal material were obvious in microscopic identification.In TLC method,spots of the same color were found on the corresponding positions of the samples and the control herbs,and there was no interference in the negative.The linear relationship of gallic acid was good in the range of 23.5ng to 705.9ng.The regression equation is Y=64795X+0.105,R^(2)=0.9999,the average recovery is 100.85%.Conclusion:The microscopic identification of stone cells(myrobalan)and starch grains(Kaempferol)was confirmed.The qualitative identification of Acorus tatarinowii by TLC is specific.The content determination method of gallic acid has strong specificity,good reproducibility,high sensitivity,simple and fast,and there is no interference to the determination of other components in the formula.Therefore,the quality standard of Mongolian medicine Wosensa Wurile has been successfully established.
作者
斯琴塔娜
唐吉思
宝山
Siqintana;TANG Ji-si;Baoshan(Inner Mongolia International Mongolian Hospital,Hohhot 010020,China)
出处
《中国民族医药杂志》
2022年第3期39-41,共3页
Journal of Medicine and Pharmacy of Chinese Minorities
基金
内蒙古自治区药品监督管理局专业技术服务(蒙药制剂规范标准制定)(项目编号:TC-19120)。
关键词
沃森萨乌日乐
质量标准
显微鉴别
薄层色谱法
高效液相色谱法
Wosensa Wurile
Quality standard
Microscopic identification
Thin layer chromatography
High performance liquid chromatography
