摘要
越来越多的研究发现一些污染物的浓度-效应曲线(CRC)呈J-型,即低浓度刺激高浓度抑制,而目前关于含有J-型CRC的污染物的联合毒性相互作用研究尚少。已有研究发现氨基甲酸酯类农药抗蚜威(PIR)对蛋白核小球藻(Chlorella pyrenoidosa)的CRC呈J型,因此,该研究采用直接均分射线法设计了4种氨基甲酸酯类农药:涕灭威(ALD)、残杀威(BAY)、呋喃丹(CAR)、灭多威(MET)分别与PIR构成的二元混合物体系:PIR-ALD(J-S型)、PIR-BAY(J-S型)、PIR-CAR(J-S型)、PIR-MET(J-S型),共20条具有不同浓度配比的射线,应用时间微板毒性分析法(t-MTA)测定二元混合物体系对C.pyrenoidosa在不同暴露时间(12、24、48、72和96 h)的生长抑制毒性,应用拓展等效线图法进行混合物组分间的毒性相互作用的定性分析。结果表明,PIR-ALD(J-S型)混合体系呈现S和J型2种浓度-效应关系,PIR-BAY、PIR-CAR、PIR-MET混合后均呈S型浓度-效应关系;随着暴露时间的延长和浓度的增加,3组二元混合物体系(PIR-ALD、PIR-CAR、PIR-MET)对C.pyrenoidosa的毒性均逐渐增强,即具有时间和浓度依赖毒性,而PIR-BAY混合体系在暴露时间内无明显毒性;依据拓展等效线图法,在暴露时间96 h时,4组二元混合体系(PIR-ALD、PIR-BAY、PIR-CAR、PIR-MET)的5条混合射线(R1、R2、R3、R4、R5)在EC_(10)、EC_(20)、EC_(30)、EC_(40)、EC_(50)效应水平下,呈现毒性相互作用,即拮抗作用或协同作用。
More and more studies have found that some pollutants have J-type concentration-response curves(CRCs),that is,low concentration stimulates and high concentration inhibits.However,there are few studies on the joint toxicity interaction of pollutants containing J-type CRC.Researches published found that pirimicarb(PIR)showed J-type on Chlorella pyrenoidosa.Therefore,four binary carbamate pesticide mixture systems(PIR-ALD,PIR-BAY,PIR-CAR and PIR-MET)of aldicarb(ALD),baygon(BAY),carbofuran(CAR),methomyl(MET)and PIR were designed by direct equipartition ray design in this study.There were 20 rays with different concentration ratios in the four mixture systems.The time-dependent microplate toxicity analysis(t-MTA)was used to determine the growth inhibition toxicity of C.pyrenoidosa at different exposure times(12,24,48,72 and 96 h).The analysis of the toxicity interaction within mixtures was carried out by using the extended isobiologram method.The results showed that PIR-ALD system presented two types of concentration-effect relationships,S-shaped and J-shaped CRC,while PIR-BAY,PIR-CAR and PIR-MET systems showed classical S-shaped CRCs.And the toxicity of the three binary mixture systems(PIR-ALD,PIR-CAR,PIR-MET)to C.pyrenoidosa increased with increase of the exposure time and concentration.That was to say,the three mixture systems(PIR-ALD,PIR-CAR,PIR-MET)had time-and concentration-dependent toxicity.But PIR-Bay mixture system showed no obvious toxicity.According to the extended isobiologram method,the four binary mixture systems exhibited toxicity interaction(antagonism or synergism)at the EC_(10)、EC_(20)、EC_(30)、EC_(40)、EC_(50) after 96 hours of exposure.
作者
周娜娜
张瑾
骆纵纵
陶梦婷
宋崇崇
卞志强
ZHOU Nana;ZHANG Jin;LUO Zongzong;TAO Mengting;SONG Chongchong;BIAN Zhiqiang(Key Laboratory of Water Pollution Control and Waste water Resource of Anhui Province,College of Environment and Energy Engineering,Anhui Jianzhu University,Hefei 230601,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2021年第11期94-100,共7页
Environmental Science & Technology
基金
国家自然科学基金(21677001)
安徽省省级质量工程项目(2020jyxm0355)
安徽建筑大学校级质量工程(2020xgk02,2020jy75)。
